AFRC IAH-CC13

88022403, Reactivity: Anti-CD1, bovine

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: Mouse x Mouse Hybridoma
• ImmunoDon: BALB/c mouse spleen
• Myelom: NS1
• Reaktivität: Anti-CD1, bovine
• Methode(n): cell culture | mammalian: suitable
• Isotyp: IgG3
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse NS1 x Mouse BALB/c spleen

Beschreibung der Zelllinie

The antibody is directed against a thymic differentiation antigen in cattle. The antigen is expressed on 50-60% of bovine thymocytes, but not on peripheral blood mononuclear cells. The antigen recognised is the bovine equivalent of CD1.

Immunogen

Bovine thymocytes

Nährmedium

RPMI 1640 + 2mM glutamine + 1% Hypoxanthine, Thymidine (HT) supplement + 10% Foetal Bovine Serum (FBS)

Subkultur-Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. These cells require mouse macrophage feeder layers upon resuscitation

Sonstige Hinweise

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