HF19

85011418, human lung, Fibroblast-like

• UNSPSC-Code: 41106514

Eigenschaften

• product name: HF19, 85011418
• Biologische Quelle: human lung
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Fibroblast-like
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human foetal lung fibroblast

Beschreibung der Zelllinie

HF19 has been established from a 14 week old female foetus. The cells are heterozygous for thymidine kinase and have been used for studies of radiation-induced DNA damage.

Anwendung

DNA repair studies

DNA-Profil

STR-PCR Data: Amelogenin: X
CSF1PO: 10,13
D13S317: 9,13
D16S539: 13,14
D5S818: 11,12
D7S820: 11,12
THO1: 6,7
TPOX: 8,11
vWA: 14,18

Nährmedium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-4x10,000 cells /cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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