C1229

Monoclonal Anti-Caspase 10 antibody produced in rat

clone 25C2, purified immunoglobulin, buffered aqueous solution

• UNSPSC Code: 12352203
• MDL number: MFCD01865513

Eigenschaften

• biological source: rat
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 25C2, monoclonal
• form: buffered aqueous solution
• mol wt: antigen (full length) 59 kDa, antigen (subunit) 17 kDa
• species reactivity: human
• technique(s): microarray: suitable, western blot: 5-10 μg/mL using a whole extract of cultured human acute T cell leukemia Jurkat cells
• isotype: IgG2a
• UniProt accession no.: Q92851
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... CASP10(843)

Beschreibung

General description

Caspases (Cysteine-requiring Aspartate protease) are a family of proteases that share similarities in amino acid sequences, structure and substrate specificity. Caspase-10 is an enzyme encoded by the CASP10 gene in humans. It is also known as Mch4, FLICE2 and ICE-LAP4 and encodes a “long” prodomain protein product of 59kDa (reported also as 55kDa). Four isoforms of caspase 10 (caspase 10a, 10b, 10c and 10d) have the same prodomain but different mature large and small subdomain. Caspase 10 undergoes a cleavage upon activation, leading to p17 and p12 subunits.

Immunogen

recombinant p17 subunit of human caspase-10.

Application

Monoclonal Anti-Caspase 10 antibody produced in rat is suitable for microarray and western blotting at a concentration of 5-10μg/mL using a whole extract of cultured human acute T cell leukemia Jurkat cells.

Biochem/physiol Actions

Caspase 10 is constitutively expressed in many fetal and adult tissues, with the lowest expression observed in brain, kidney, prostate, testis, and colon. Caspase 10 contains two death effector domains (DEDs) involved in the linking to the death effector domain of the adapter protein FADD and recruiting the complex to TNFR1 and Fas. Caspase 10 is one of the earliest caspases involved in the apoptosis induced cascade and may be responsible for the activation of most of the other caspases including caspase 3, 4, 6, 7 ,8 and 9.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.