B8894

Albumin, acetyliert aus Rinderserum

protease free, Molecular Biology

• Synonym(e): Ac-BSA, Acetyl BSA, Acetyliertes BSA
• CAS-Nummer: 9048-46-8
• UNSPSC Code: 12352202
• EC Number: 232-936-2
• NACRES: NA.27
• MDL number: MFCD01114300
• eCl@ss: 32160409
• Biological source: bovine
• Form: solution
• Technique(s): FISH: suitable
• Impurities: HIV I and HIVII, HCV and HBsAg, tested negative
• Concentration: 20 mg/mL in H₂O

Eigenschaften

• biological source: bovine
• Quality Segment: 200
• grade: Molecular Biology
• product line: BioReagent
• form: solution
• purified by: heat shock fractionation
• contains: does not contain stabilizer or preservative
• origin: USA origin
• concentration: 20 mg/mL in H₂O
• technique(s): FISH: suitable
• impurities: HIV I and HIVII, HCV and HBsAg, tested negative
• UniProt accession no.: P02769
• foreign activity: DNase free , DNase, RNase, NICKase and protease, none detected, NICKase free , Protease free , RNase free
• storage temp.: −20°C
• Gene Information: bovine ... ALB(280717)

Beschreibung

General description

Serum albumin is the most extensive plasma protein in the circulatory system. It is produced within the liver and exported as a non-glycosylated protein to the blood plasma. The molecular weight of native serum albumin is around 66 kDa. Native serum albumin is a negatively charged protein.

Acetylation of bovine serum albumin particularly modifies lysine (Lys) residues with elevated frequency, with lesser degrees of modification of serine (Ser) and threonine (Thr) residues. MALDI-TOF mass spectrometry analysis of acetylated BSA has indicated an increased MW of about 3000, compared to native BSA (Ostatná, V. et al., J. Electroanalytical Chem., 821, 97-103 (2018)).. This acetylation process also derivatizes similar amino acid residues in any trace contaminating nucleases in the BSA, to inactivate that nuclease activity. This makes acetylated BSA useful in molecular biology applications where trace nuclease activity must be minimized.

Application

Acetylated BSA has been used as a component of the reaction mixture for adapter ligation. In addition, this B8894 acetylated BSA product has been used in other applications, such as:

• In cDNA library preparation (Hafner, M. et al., RNA, 17(9), 1697-1712 (2011))
• As a blocking agent with streptavidin beads used for capture of biotinylated RNA (Winz, M.-L. et al., Nat. Protocol., 12(1), 122-149 (2017))

Acetyliert zur Inaktivierung von Nukleasen, die gemeinhin in BSA vorkommen. Acetyliertes BSA sollte nicht als Proteinstandard genutzt werden, da die Acetylierung der Tyrosinreste im Lowry-Assay und in ähnlichen Proteinnachweistests eine Farbentwicklung verhindert.

Select the right albumin for your application with the Albumin Selection Guide.

Biochem/physiol Actions

Native serum albumin plays a major role in maintaining the pH and osmotic pressure of the blood. It is also involved in transporting a diverse range of endogenous and exogenous metabolites, such as amino acids, fatty acids, drugs, steroids and metal ions. Native serum albumin can have a role in modulating the levels of Ca²⁺ and Mg²⁺ metals in blood.

Features and Benefits

This acetylated BSA product has been tested for the following properties:

• DNase-free / Exonuclease-free
• Nickase-free / Endonuclease-free
• Protease-free
• RNase-free

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)