B2059

Biotin-Polyethylenoxid-Jodoacetamid

• Synonym(e): Biotin PEO Iodoacetamide
• Empirische Formel (Hill-System): C₁₈H₃₁IN₄O₅S
• Molekulargewicht: 542.43
• MDL-Nummer: MFCD04974830
• UNSPSC-Code: 12352119
• PubChem Substanz-ID: 24891627
• NACRES: NA.32

Eigenschaften

• Beschreibung: Sulfhydryl specific, water soluble biotinylation reagent
• Form: powder
• Löslichkeit: H₂O: 10 mg/mL
• Lagertemp.: 2-8°C
• SMILES String: ICC(=O)NCCOCCOCCNC(=O)CCCCC1SCC2NC(=O)NC12
• InChI: 1S/C18H31IN4O5S/c19-11-16(25)21-6-8-28-10-9-27-7-5-20-15(24)4-2-1-3-14-17-13(12-29-14)22-18(26)23-17/h13-14,17H,1-12H2,(H,20,24)(H,21,25)(H2,22,23,26)
• InChIKey: LWGLKVINXNMNJX-UHFFFAOYSA-N

Beschreibung

Allgemeine Beschreibung

Biotin polyethyleneoxide (PEO) iodoacetamide is a sulfhydryl (thiol) specific biotinylation reagent.

Anwendung

Biotin Polyethyleneoxide Iodoacetamide has been used as a thiol reactive reagent to demonstrate the presence of free thiols in capsid protein L1 of human papillomavirus 16. It has been used for biotinylation of proteins for ELISA (enzyme-linked immunosorbent assay) competition and pull down experiments.

• Beinhaltet einen 12-Atom hydrophilen Spacer.
• bei pH 7.5 - 8.5 typischerweise an Sulfhydrylgruppen gekoppelt.
• Besonders nützlich für Cystein-labeling in vielen Proteomik Anwendungen wie Peptid-Mapping und Massenspektrometrie.

Biochem./physiol. Wirkung

Biotin polyethyleneoxide (PEO) iodoacetamide group reacts specifically with reduced thiols at pH 7.5 - 8.5. Peptides and small molecules containing thiol groups may also be labeled using this reagent. The biotin PEO iodoacetamide enables tagging of cysteine residues and conjugation to sulfhydryls in proteins making it useful in proteomics, peptide mapping, phosphopeptide analysis, and mass spectrometry.

Sicherheitshinweise

• Piktogramme: GHS07
• Signalwort: Warning
• H-Sätze: H315,H319,H335
• P-Sätze: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Gefahreneinstufungen: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Zielorgane: Respiratory system
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: dust mask type N95 (US), Eyeshields, Gloves