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A9415
Actinomycin D
from Streptomyces sp., suitable for cell culture, ≥95%
Synonym(e):
Actinomycin C1, Actinomycin IV, Dactinomycin
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InChI key
RJURFGZVJUQBHK-IIXSONLDSA-N
InChI
1S/C62H86N12O16/c1-27(2)42-59(84)73-23-17-19-36(73)57(82)69(13)25-38(75)71(15)48(29(5)6)61(86)88-33(11)44(55(80)65-42)67-53(78)35-22-21-31(9)51-46(35)64-47-40(41(63)50(77)32(10)52(47)90-51)54(79)68-45-34(12)89-62(87)49(30(7)8)72(16)39(76)26-70(14)58(83)37-20-18-24-74(37)60(85)43(28(3)4)66-56(45)81/h21-22,27-30,33-34,36-37,42-45,48-49H,17-20,23-26,63H2,1-16H3,(H,65,80)(H,66,81)(H,67,78)(H,68,79)/t33-,34-,36+,37+,42-,43-,44+,45+,48+,49+/m1/s1
SMILES string
CC(C)[C@H]1NC(=O)[C@@H](NC(=O)c2ccc(C)c3OC4=C(C)C(=O)C(N)=C(C(=O)N[C@H]5[C@@H](C)OC(=O)[C@H](C(C)C)N(C)C(=O)CN(C)C(=O)[C@@H]6CCCN6C(=O)[C@H](NC5=O)C(C)C)C4=Nc23)[C@@H](C)OC(=O)[C@H](C(C)C)N(C)C(=O)CN(C)C(=O)[C@@H]7CCCN7C1=O
biological source
Streptomyces sp.
assay
≥95%
form
powder
technique(s)
cell culture | mammalian: suitable
mp
251-253
solubility
ethanol, DMSO: soluble (Stable in aqueous solutions at 2-8 °C.)
antibiotic activity spectrum
neoplastics
mode of action
DNA synthesis | interferes
storage temp.
2-8°C
Quality Level
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Verwandte Kategorien
1 of 4
Dieser Artikel | |||
|---|---|---|---|
| antibiotic activity spectrum neoplastics | antibiotic activity spectrum neoplastics | antibiotic activity spectrum neoplastics | antibiotic activity spectrum - |
| mode of action DNA synthesis | interferes | mode of action DNA synthesis | interferes | mode of action DNA synthesis | interferes | mode of action - |
| technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) - | technique(s) - |
| Quality Level 300 | Quality Level 200 | Quality Level 300 | Quality Level - |
| form powder | form powder | form powder | form - |
| assay ≥95% | assay ≥95% (HPLC) | assay ~98% (HPLC) | assay - |
General description
Application
Biochem/physiol Actions
Preparation Note
Other Notes
Disclaimer
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 2 Oral - Carc. 1B - Repr. 1B
Lagerklasse
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges
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Artikel
Die Abtötungskurve von Antibiotika ist ein Dosis-Wirkungs-Versuch, in dem Säugerzellen zunehmenden Mengen eines selektiven Antibiotikums ausgesetzt werden
Antibiotic kill curve is a dose response experiment in which mammalian cells are subjected to increasing amounts of selection antibiotic
Protokolle
MTT-Assayprotokoll für die Messung von Zellviabilität, Zellproliferation und Zytotoxizität. Anwendungshinweise für die MTT Reagenzvorbereitung und Beispiele für Anwendungen.
Durchführung nicht-radioaktiver Quantifizierung von Zellproliferation, Viabilität und Zytotoxizität adhärenter oder Suspensionszellen, die in 96-Well-Mikrotiterplatten kultiviert werden, mittels colorimetrischer Assays.
WST-1-Assayprotokoll für die Messung von Zellviabilität, Zellproliferation, Aktivierung und Zytotoxizität. Anwendungshinweise für die WST-1 Reagenzvorbereitung und Beispiele für Anwendungen. Häufig gestellte Fragen und Anleitung für die Fehlersuche für den WST-1 Assay
MTT assay protocol for measuring cell viability, proliferation and cytotoxicity. Instructions for MTT reagent preparation and examples of applications.
Verwandter Inhalt
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How can I determine the shelf life / expiration / retest date of this product?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How long are stock solutions of Actinomycin D (Product No. A9415) stable?
1 answer-
Stock solutions of this product in DMSO or ethanol should be stored in foil-wrapped vials at -20 °C. In DMSO, solution can be stored for up to 1 month at -20 °C. Do not store in glass vials.
Helpful?
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How do you recommend dissolving Product No. A9415 (Actinomycin D)?
1 answer-
Stock solutions of this product may be prepared at 10 mg/mL in either ethanol (EtOH) or dimethyl sulfoxide (DMSO).
Helpful?
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What is the working concentration for Actinomycin D (Product No. A9415)?
1 answer-
For cell culture applications, actinomycin D is used as a selection agent and is used in banding techniques to differentiate between different regions of chromosomes. Suggested working concentrations is 1 μg/mL.
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