A4487

Aphidicolin, Ready Made Solution

from Nigrospora sphaerica

• Synonym(e): ICI 69653, NSC-234714
• Empirische Formel (Hill-System): C₂₀H₃₄O₄
• CAS-Nummer: 38966-21-1
• Molekulargewicht: 338.48
• EG-Nummer: 200-664-3
• MDL-Nummer: MFCD00083214
• UNSPSC-Code: 51102829
• NACRES: NA.85

Eigenschaften

• Biologische Quelle: Nigrospora sphaerica
• Qualitätsniveau: 200
• Dampfdruck: 0.55 hPa ( 20 °C)
• Form: liquid
• Farbe: clear colorless
• Löslichkeit: H₂O: miscible (completely)
• Wirkungsspektrum von Antibiotika: neoplastics; viruses
• Wirkungsweise: DNA synthesis | interferes; enzyme | inhibits
• Lagertemp.: −20°C
• SMILES String: O[C@]1([C@H]2C[C@@]3([C@@]4([C@H]([C@@]([C@@H](CC4)O)(CO)C)CC[C@H]3C2)C)CC1)CO
• InChI: 1S/C20H34O4/c1-17(11-21)15-4-3-13-9-14-10-19(13,7-8-20(14,24)12-22)18(15,2)6-5-16(17)23/h13-16,21-24H,3-12H2,1-2H3/t13-,14+,15-,16+,17-,18-,19-,20-/m0/s1
• InChIKey: NOFOAYPPHIUXJR-APNQCZIXSA-N

Beschreibung

Allgemeine Beschreibung

Aphidicolin is a tetracyclic diterpene with antiviral and antimitotic properties used for cell cycle synchronization in various cell lines.[1]

Anwendung

Aphidicolin, from Nigrospora sphaerica, was used for cell cycle synchronization of tobacco BY-2 cells. It is used to block the entry of cells into S-phase.[2]

Biochem./physiol. Wirkung

Aphidicolin specifically inhibits DNA polymerase α that is responsible for DNA replication. It also inhibits α-like DNA polymerases of plants and yeasts but does not inhibit synthesis of RNA and proteins. Aphidicolin competes for the dCTP-specific binding site on DNA polymerase α specifically. Aphidicolin has antiviral and antimitotic properties. It blocks the cell cycle at S phase. It has been shown to induce apoptosis in HeLa cells.[3]

Verpackung

1 ml

Physikalische Form

Aphidicolin Ready Made Solution supplied as a 1 mg/ml solution in DMSO.

Sonstige Hinweise

Keep container tightly closed in a dry and well-ventilated place. Storage class (TRGS 510): Combustible liquids

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): 188.6 °F - closed cup
• Flammpunkt (°C): 87 °C - closed cup