I accidentally bought this thinking it was MTT salt to use in a cell proliferation assay but it is MTT Formazan, the water insoluble end product! Therefore, it is useless for the assay. I suppose you could use it as a control to optimize your buffer for solubilizing the insoluble Formazan. The description for this product is misleading and should clarify that this is not what you need for an MTT assay, and refer the customer to MTT salt. That said, this is a nice purple crystalline powder and insoluble in water consistent with MTT Formazan, if that is what you actually want.
Alle Fotos(1)
Wichtige Dokumente
88417
Thiazolylblau-formazan
MTT Formazan
MTT reduction end product, powder
Synonym(e):
1-(4,5-Dimethylthiazol-2-yl)-3,5-diphenylformazan, MTT-Formazan
Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Größe auswählen
250 MG
€ 49,40
1 G
€ 174,00
€ 49,40
Check Cart for Availability
Alle Fotos(1)
Größe auswählen
Ansicht ändern
250 MG
€ 49,40
1 G
€ 174,00
About This Item
Empirische Formel (Hill-System):
C18H17N5S
CAS-Nummer:
Molekulargewicht:
335.43
MDL-Nummer:
UNSPSC-Code:
12352204
PubChem Substanz-ID:
NACRES:
NA.83
€ 49,40
Check Cart for Availability
Empfohlene Produkte
Produktbezeichnung
Thiazolylblau-formazan, MTT reduction end product
Form
powder
Löslichkeit
chloroform: 10 mg/mL, clear, dark violet
SMILES String
Cc1nc(\N=N\C(=N\Nc2ccccc2)c3ccccc3)sc1C
InChI
1S/C18H17N5S/c1-13-14(2)24-18(19-13)23-22-17(15-9-5-3-6-10-15)21-20-16-11-7-4-8-12-16/h3-12,20H,1-2H3/b21-17+,23-22+
InChIKey
GIFKVQOPAJDJLO-SZVLODPRSA-N
Suchen Sie nach ähnlichen Produkten? Aufrufen Leitfaden zum Produktvergleich
Biochem./physiol. Wirkung
MTT assay is a colorimetric reaction, generally used to examine cell viability. It involves the enzymatic reduction of a yellow-colored, water-soluble, 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) to purple-colored, water-insoluble, MTT-formazan. This reaction is catalyzed by mitochondrial dehydrogenase and only occurs in viable cells.[4][5] Thus, the amount of blue formazan is proportional to the number of viable cells. The absorbance of the formazan solution is analyzed spectrophotometrically at 570 nm.[6]
Sonstige Hinweise
Reviews: Tetrazolium salts and formazans[7]
Ähnliches Produkt
Produkt-Nr.
Beschreibung
Preisangaben
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Gloves, type N95 (US)
Hier finden Sie alle aktuellen Versionen:
Besitzen Sie dieses Produkt bereits?
In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
Kunden haben sich ebenfalls angesehen
Chao Zhang et al.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 80, 253-259 (2016-05-03)
Costunolide, an active sesquiterpene lactone, is derived from many herbal medicines and it exhibits a broad spectrum of bioactivities such as anti-inflammatory, potential anti-tumor activity. Herein we assessed the anti-cancer effects of costunolide on U2OS cells and explored the underlying
Jianing Li et al.
Pathology, research and practice, 215(10), 152591-152591 (2019-08-26)
The role of transforming growth factor beta (TGF-β) in lung cancer is well known. TGF-β-mediated cellular proliferation and angiogenesis through similar to mothers against decapentaplegic homolog 2 (Smad2) protein has also been well elucidated. Smad2 is a predicted target for
Primary cultures of cardiac myocytes as in vitro models for pharmacological and toxicological assessments
In Vitro Methods in Pharmaceutical Research, 209-223 (1997)
Nanotoxicology: evaluating toxicity potential of drug-nanoparticles
Nanoparticulate drug delivery, 1, 123-155 (2012)
Polymeric nanoparticles for the selective therapy of inflammatory bowel disease
Test, 508, 377-397 (2012)
Active Filters
Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..
Setzen Sie sich mit dem technischen Dienst in Verbindung.
