If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
Fortfahren mit
77617
Phenol–Chloroform–Isoamylalkohol-Mischung
BioUltra, Molecular Biology, 25:24:1
Größe auswählen
| Ihnen/SKU | Verfügbarkeit | Preis |
|---|---|---|
100 mL | Warenkorb auf Verfügbarkeit prüfen | € 117,00 |
500 mL | Warenkorb auf Verfügbarkeit prüfen | € 393,00 |
Über diesen Artikel
grade
Molecular Biology
Quality Segment
product line
BioUltra
form
liquid
technique(s)
ChIP: suitable, DNA extraction: suitable, DNA purification: suitable
impurities
DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected
pH
7.2-8.3(H2O-phase, after extraction with H2O, 1:1)
density
1.28 g/mL at 20 °C
absorption
cut-off at 360 nm
suitability
suitable for molecular biology
application(s)
agriculture
storage temp.
2-8°C
SMILES string
ClC(Cl)Cl.CC(C)CCO.Oc1ccccc1
InChI
1S/C6H6O.C5H12O.CHCl3/c7-6-4-2-1-3-5-6;1-5(2)3-4-6;2-1(3)4/h1-5,7H;5-6H,3-4H2,1-2H3;1H
InChI key
ZYWFEOZQIUMEGL-UHFFFAOYSA-N
General description
Das PCI-Gemisch ist vielseitig einsetzbar, so auch für die RNA-Isolierung, in der es effizient RNA aus tierischen und pflanzlichen Geweben für nachfolgende Analysen wie reverse Transkription und Genexpressionsstudien extrahiert. Darüber hinaus ist das PCI-Gemisch auch bei der DNA-Extraktion von entscheidender Bedeutung und ermöglicht eine effiziente Rückgewinnung für verschiedene Anwendungen wie DNA-Minisatelliten-Fingerprinting für die genetische Identifizierung. Als allgemeine Aufreinigungstechnik gewährleistet das PCI-Gemisch die Gewinnung von DNA- und RNA-Präparationen hoher Qualität für die weitere Forschung.
Application
Biochem/physiol Actions
Features and Benefits
- Vielseitig und anpassbar an eine Vielzahl von Labor- und Forschungsanwendungen
- Frei von DNase, RNase, NICKase und Protease
Physical form
Other Notes
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| Quality Level 200 | Quality Level 100 | Quality Level 100 | Quality Level 200 |
| application(s) agriculture | application(s) - | application(s) - | application(s) - |
| grade Molecular Biology | grade Molecular Biology | grade Molecular Biology | grade Molecular Biology |
| technique(s) ChIP: suitable, DNA purification: suitable, DNA extraction: suitable | technique(s) - | technique(s) - | technique(s) GC/GC: suitable |
| form liquid | form liquid | form liquid | form liquid |
| impurities DNases, none detected, phosphatases, none detected, RNases, none detected, proteases, none detected | impurities DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected | impurities DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected | impurities DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected, ≤0.001% free alkali (as NH3) |
Still not finding the right product?
Explore all of our products under Phenol–Chloroform–Isoamylalkohol-Mischung
signalword
Danger
Hazard Classifications
Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2 - STOT SE 3
target_organs
Central nervous system, Liver,Kidney, Nervous system,Kidney,Liver,Skin
Lagerklasse
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
flash_point_f
174.2 °F
flash_point_c
79 °C
ppe
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
Zulassungslistungen
Zulassungslistungen werden hauptsächlich für chemische Produkte erstellt. Für nicht-chemische Produkte können hier nur begrenzte Angaben gemacht werden. Kein Eintrag bedeutet, dass keine der Komponenten gelistet ist. Es liegt in der Verantwortung des Benutzers, die sichere und legale Verwendung des Produkts zu gewährleisten.
67-66-3
Hier finden Sie alle aktuellen Versionen:
Besitzen Sie dieses Produkt bereits?
In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
-
How can I determine the shelf life / expiration / retest date of this product?
1 answer-
Helpful?
-
-
Hello, Is this product saturated with Tris-Hcl?
1 answer-
Yes, Tris HCl is used to prepare the 100 mM Tris pH 8.0 saturation buffer.
Helpful?
-
-
Is the packaging configuration on the 100 ml presentation glass, amber bottle?
1 answer-
Yes, this product is packaged in an amber glass bottle.
Helpful?
-
-
Dans la description, il est dit que le produit est "composé de 25 % de phénol, 24 % de chloroforme et 1 % d'alcool isoamylique". Les 50% restant, qu'est-ce que c'est ou est-ce une erreur ?
1 answer-
As reported in the 'Physical Form' section, under 'DESCRIPTION', the remainder of this material is 100 mM TRIS, pH 8.0, containing approximately 0.1% 8-hydroxyquinoline.
Helpful?
-
-
How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
Helpful?
-
-
Is there an overlay layer of aqueous TE buffer in the bottles?
1 answer-
The product is saturated with TRIS-buffer. At the time of filling the product consists of a single phase. It is possible that an aqueous phase will be liberated, particularly at lower temperatures. Should phase separation occur, the upper will be the aqueous Tris.
Helpful?
-