66540453

EDi012-B

Human iPS Cell Line

• Synonym(e): EBiSC iPSC Line, Human iPSC, Induced Pluripotent Stem Cell Line, iPS Cell, iPSC
• UNSPSC-Code: 41106509

Eigenschaften

• Biologische Quelle: human dermis (fibroblast)
• Verfahren zur Umprogrammierung: Sendai virus
• Beschreibung: age (N/A)
• Hersteller/Markenname: EBiSC^™
• Geschlecht: female
• Wachstumsmodus: adherent (pluripotent)
• Methode(n): cell culture | stem cell: suitable
• Relevante Krankheit(en): none
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Allgemeine Beschreibung

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.

The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.

Ursprung der Zelllinie

Depositor
University of Edinburgh

Beschreibung der Zelllinie

Derivation
Primary cell type: Fibroblast of dermis
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Other:From the same donor: EDi012-A, EDi012-C

Characterization
Analysis of Undifferentiated Cells
Marker expression: POU5F1 (OCT-4)(+)SSEA-4 (+)TRA 1-60 (+) SSEA-1 (-)

Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Not done
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: -

Genotyping
Karyotyping
Passage number: 28
Cell line karyotype: No autosomal or sex chromosome aneuploidies detected
Karyotyping method: BACs-on-Beads

Verlinkung

• Additional Cell Line Info
• EBiSC Access Use Agreement (EAUA)
• Cell Line Information Pack (CLIP)

Note: EAUA and CLIP must be completed before order fulfillment

Subkultur-Routine

Surface coating: Matrigel^®/Geltrex^®
Passage method: Enzyme-free cell dissociation / EDTA
O₂ concentration: 20%
CO₂ concentration: 5%
Culture medium: Essential E8^®

Rechtliche Hinweise

E8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation

EBiSC is a trademark of Fraunhofer-Gesellschaft

GELTREX is a registered trademark of Life Technologies Corporation

Matrigel is a registered trademark of Corning, Inc.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable