62305

Lipase aus Rhizopus oryzae

powder (fine), ~10 U/mg

• Synonym(e): Lipase aus Rhizopus arrhizus, Triacylglycerol-acylhydrolase, Triacylglycerol-lipase
• CAS-Nummer: 9001-62-1
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-619-9
• EG-Nummer: 3.1.1.3 (BRENDA, IUBMB)
• MDL number: MFCD00131509
• Specific activity: ~10 U/mg

Eigenschaften

• form: powder (fine)
• Quality Segment: 100
• specific activity: ~10 U/mg
• mol wt: M_r ~43000
• storage temp.: 2-8°C
• InChI: 1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
• InChI key: QWZUIMCIEOCSJF-CHHCPSLASA-N

Beschreibung

General description

Research Area: Cell Signaling
Rhizopus oryzae lipase (ROL) is a protein synthesized in a precursor form that includes a presequence of 26 amino acids, followed by a prosequence of 97 amino acids, which is attached to the N-terminal of a mature sequence consisting of 269 amino acids.

Lipase from Rhizopus oryzae (ROL) comprises an oxyanion hole, four N-glycosylation sites, and an active site region. It possesses N-terminal presequence and prosequence.

Application

Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

Lipase from Rhizopus oryzae has been used:

• to test its effect on 1,2-diolein synthesis and triolein ethanolysis
• for immobilization on graphene oxide support for biocatalysis studies
• to digest triglycerides (TAG) from Chlamydomonas reinhardtii and S. cerevisiae[1]

Biochem/physiol Actions

Rhizopus oryzae lipase (ROL) has been extensively researched for its regiospecificity in biodiesel production. Due to its remarkable characteristics, including 1,3-specificity, high enantioselectivity, and stability in organic solvents, ROL has garnered significant attention for applications in the energy, food, and pharmaceutical industries.

Tri-, Di-, und Monoglyceride sind hydrolysiert (in abnehmender Ratenreihenfolge).

Lipase from Rhizopus oryzae (ROL) acts as a catalyst for the enzymatic biosynthesis of polyglycerol polyricinoleate through a reversal of hydrolysis.[2] ROL is useful in the industrial production of structured lipids due to its 1,3-regiospecificity functionality.

Other Notes

1 U entspricht der Enzymmenge, die 1 μmol Buttersäure pro Minute bei pH 8.0 und 40°C freisetzt (Tributyrin, Fluka^®-Nr. 91010 als Substrat) 5000 U wie oben beschrieben entsprechen ~1 U, wenn Triolein (Fluka Nr. 62314) als Substrat, bei pH 8.0 und 40°C, verwendet wird

Catalyst for the interesterification of oils and fats; For removal of interfering triglycerides in the electroimmunoassay of apolipoprotein B; Racemic epoxy ester resolution through enantioselective enzymatic hydrolysis.

Note: When triacetin is used as substrate, the pH is 7.4. Incubation time: 60 minutes.

Sicherheitshinweise

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)