62310

Lipase aus Rhizopus niveus

powder (fine), ≥1.5 U/mg

• Synonym(e): Lipase N, Triacylglycerol-acylhydrolase, Triacylglycerol-lipase
• CAS-Nummer: 9001-62-1
• UNSPSC Code: 12352204
• NACRES: NA.26
• EC Number: 232-619-9
• EG-Nummer: 3.1.1.3 (BRENDA, IUBMB)
• MDL number: MFCD00131509
• Specific activity: ≥1.5 U/mg
• Biological source: fungus (Rhizopus niveus)

Eigenschaften

• biological source: fungus (Rhizopus niveus)
• Quality Segment: 100
• form: powder (fine)
• specific activity: ≥1.5 U/mg
• mol wt: M_r ~83000
• storage temp.: 2-8°C
• InChI: 1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
• InChI key: QWZUIMCIEOCSJF-CHHCPSLASA-N

Beschreibung

General description

Lipases hydrolyze ester bonds of triacylglycerols and in some conditions, catalyze the synthesis of ester bonds by transesterification. Lipase from Rhizopus niveus exists as a single polypeptide chain with a molecular mass of about 30-34kDa.

Application

Lipase from Rhizopus niveus has been used to determine the positional distribution of fatty acids within triacylglycerol by hydrolysis of the acyl ester linkage at C-1 and C-3. It has also been used to induce intragastric lipolysis and study its effects on felodipine (a medication) release from a hydrophilic, extended release tablet.

Biochem/physiol Actions

Lipase from Rhizopus niveus is specifically used to produce cocoa butter substitutes.

Tri-, Di-, und Monoglyceride sind hydrolysiert (in abnehmende Ratenreihenfolge).

Other Notes

Substrate specificity: preferentially hydrolyzes the 1- and 3-positions(middle to long fatty acids); optimum pH 5.0-7.0; optimum temperature 30-45°C; Selective enzymatic removal of protecting functions (C-terminal COOH function)

1 U entspricht der Enzymmenge, die 1 μmol Fettsäure aus Triglyceriden pro Minute bei pH 7.7 und 37°C freisetzt (Olivenöl als Substrat); 300 U wie oben beschrieben entsprechen ~1 U, wenn Triolein, Fluka^®-Nr. 62314 bei pH 8.0 und 40°C als Substrat verwendet wird.

Note: When triacetin is used as substrate, the pH is 7.4. Incubation time: 60 minutes.

Sicherheitshinweise

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)