62310

Lipase aus Rhizopus niveus

powder (fine), ≥1.5 U/mg

• Synonym(e): Lipase N, Triacylglycerol-acylhydrolase, Triacylglycerol-lipase
• CAS-Nummer: 9001-62-1
• EC-Nummer: 3.1.1.3 (BRENDA, IUBMB)
• EG-Nummer: 232-619-9
• MDL-Nummer: MFCD00131509
• UNSPSC-Code: 12352204
• NACRES: NA.26

Eigenschaften

• Biologische Quelle: fungus (Rhizopus niveus)
• Qualitätsniveau: 100
• Form: powder (fine)
• Spezifische Aktivität: ≥1.5 U/mg
• Mol-Gew.: M_r ~83000
• Lagertemp.: 2-8°C
• InChI: 1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
• InChIKey: QWZUIMCIEOCSJF-CHHCPSLASA-N

Beschreibung

Allgemeine Beschreibung

Lipases hydrolyze ester bonds of triacylglycerols and in some conditions, catalyze the synthesis of ester bonds by transesterification. Lipase from Rhizopus niveus exists as a single polypeptide chain with a molecular mass of about 30-34kDa.

Anwendung

Lipase from Rhizopus niveus has been used to determine the positional distribution of fatty acids within triacylglycerol by hydrolysis of the acyl ester linkage at C-1 and C-3. It has also been used to induce intragastric lipolysis and study its effects on felodipine (a medication) release from a hydrophilic, extended release tablet.

Biochem./physiol. Wirkung

Lipase from Rhizopus niveus is specifically used to produce cocoa butter substitutes.

Tri-, Di-, und Monoglyceride sind hydrolysiert (in abnehmende Ratenreihenfolge).

Einheitendefinition

1 U entspricht der Enzymmenge, die 1 μmol Fettsäure aus Triglyceriden pro Minute bei pH 7.7 und 37°C freisetzt (Olivenöl als Substrat); 300 U wie oben beschrieben entsprechen ~1 U, wenn Triolein, Fluka^®-Nr. 62314 bei pH 8.0 und 40°C als Substrat verwendet wird.

Sonstige Hinweise

Note: When triacetin is used as substrate, the pH is 7.4. Incubation time: 60 minutes.

Substrate specificity: preferentially hydrolyzes the 1- and 3-positions(middle to long fatty acids); optimum pH 5.0-7.0; optimum temperature 30-45°C; Selective enzymatic removal of protecting functions (C-terminal COOH function)

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• H-Sätze: H334
• P-Sätze: P261 - P284 - P501
• Gefahreneinstufungen: Resp. Sens. 1
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)