61305

Penicillinase aus Bacillus cereus

lyophilized, powder, white, ~13 U/mg

• Synonym(e): β-Lactamase I aus Bacillus cereus, β-Lactamase II, Cephalosporinase, Penicillin-amido-β-lactam-Hydrolase
• CAS-Nummer: 9073-60-3
• UNSPSC Code: 12352204
• NACRES: NA.54
• EG-Nummer: 3.5.2.6 (BRENDA, IUBMB)
• MDL number: MFCD00131819
• Specific activity: ~13 U/mg
• Biological source: Bacillus sp. (Bacillus cereus)

Eigenschaften

• biological source: Bacillus sp. (Bacillus cereus)
• Quality Segment: 100
• form: powder
• quality: lyophilized
• specific activity: ~13 U/mg
• mol wt: M_r ~30000
• technique(s): activity assay: suitable
• color: white
• suitability: suitable for component for culture media
• application(s): diagnostic assay manufacturing
• foreign activity: β-lactamase II ≤2%
• storage temp.: −20°C

Beschreibung

General description

Penicillinase from Bacillus cereus is both an extracellular and cell-bound enzyme. It has a single polypeptide chain and has a molecular weight of 31 kDa. It lacks cysteine in the protein sequence.

Application

Wird bei der Herstellung von Penicillin verwendet.

Penicillinase from Bacillus cereus may be used in the preparation of biosensor for amperometric detection of hydrolysis of penicillin.

Biochem/physiol Actions

Penicillinase katalysiert speziell die Hydrolyse des β-Lactam-Rings von Penicillin.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Eine Einheit hydrolisiert 1,0 μmol des angegebenen Substrats pro Minute bei pH-Wert 7,0 und 25 °C. Als Benzylpenicillin-Einheiten vertrieben. Diese internationale Einheit (mit Benzylpenicillin als Substrat) entspricht ungefähr 600 Levy- bzw. 75 Pollock-Einheiten.

Used to destroy β-lactam antibiotics in body fluids or culture media. For the determination of penicillin; In the coupled enzyme assay for isopenicillin N synthetase; ELISA of estradiol; Penicillinase optodes.

Sicherheitshinweise

• pictograms: GHS08
• signalword: Danger
• hcodes: H317,H334
• pcodes: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Faceshields, Gloves