52944

Anti-Kaninchen IgG−Abberior^® STAR 488 in Ziege hergestellte Antikörper

for STED application

• Synonym(e): Abberior^® STAR 488-Anti-Kaninchen IgG in Ziege hergestellte Antikörper
• NACRES: NA.32
• UNSPSC Code: 12352200

Eigenschaften

• biological source: goat
• Quality Segment: 100
• antibody form: affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• species reactivity: rabbit
• availability: available only in USA and Canada
• concentration: ~1 mg/mL
• fluorescence: λ_ex 503 nm; λ_em 524 nm in PBS, pH 7.4
• storage temp.: −20°C

Beschreibung

General description

Abberior STAR 488 was developed for STED and confocal microscopy in the green spectral region. It is a bright green fluorescent dye. The dye works extremely well with Abberior Instruments Expert Line Microscopes and Leica STED microscopes. It can be very effectively excited with the prominent 488 nm laser line. For STED microscopy, Abberior STAR 488 can be efficiently used with STED Laser wavelengths between 590 - 610 nm. Abberior STAR 488 can substitute dyes like Oregon Green 488, Atto^™ 488 or Alexa Fluor^™ 488. Abberior STAR 488 is the ideal partner for Abberior STAR 440SXP to obtain optimal 2 Color STED results. Best results are obtained with freshly prepared samples.

Photophysical properties (carboxylic acid):
Absorption Maximum, λ_ex [nm]: 503 (PBS pH 7.4), 504 (H₂O), 507 (MeOH + 0.1% TFA)
Extinction Coefficient, ε_max [M^-1cm^-1]: 65 000 (PBS pH 7.4), 80 000 (H₂O), 85 000 (MeOH + 0.1% TFA)
Correction Factor, CF₂₆₀ = ε₂₆₀/ε_max: 0,28 (PBS pH 7.4)
Correction Factor, CF₂₈₀ = ε₂₈₀/ε_max: 0,14 (PBS pH 7.4)
Fluorescence Maximum, λ_em [nm]: 524 (PBS pH 7.4), 525 (H₂O), 531 (MeOH + 0.1% TFA)
Recommended STED Wavelength, λ [nm]: 590 - 610
Fluorescence Quantum Yield, λ: 0,89 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 3,9 (PBS pH 7.4)

Application

Designed and tested for fluorescent super-resolution microscopy

Anti-Rabbit IgG-Abberior^® STAR 488 antibody has been used as a secondary antibody:

• for fluorescent immunohistochemistry in prostate cancer cell lines, PPC-1 and TSU-Pr1
• for immunofluorescence in HeLa and monkey kidney COS-7 cells
• for STED (stimulated emission depletion) microscopy in OLN-t40-α-syn cells (oligodendroglial cells)

Analysis Note

May contain significant amounts of BSA as stabilizer
unconjugated dye ≤5% of total fluorescence

Other Notes

Find more infomation here Abberior^®

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Atto is a trademark of Atto-Tec GmbH

abberior is a registered trademark of Abberior GmbH

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable