41155

Anti-Kaninchen IgG−Abberior^® CAGE 500 in Ziege hergestellte Antikörper

for single-molecule switching microscopy (e.g. PALM, STORM, GSDIM)

• Synonym(e): Abberior^® CAGE 500P-Anti-Kaninchen IgG in Ziege hergestellte Antikörper
• UNSPSC-Code: 12352200
• NACRES: NA.45

Eigenschaften

• Biologische Quelle: goat
• Qualitätsniveau: 100
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: secondary antibodies
• Klon: polyclonal
• Form: buffered aqueous solution
• Speziesreaktivität: rabbit
• Verfügbarkeit: available only in USA and Canada
• Konzentration: ~1 mg/mL
• Fluoreszenz: λ_ex 511 nm; λ_em 525 nm in PBS, pH 7.4 (photoactivated)
• Eignung: corresponds for SDS-PAGE
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Abberior CAGE 500 is a caged dye which is initially colorless and nonfluorescent. When illuminated with UV light it undergoes a rapid uncaging reaction and releases a highly fluorescent dye which fluoresces at 511 nm.
Abberior CAGE 500 is designed for single-molecule photoswitching microscopy modes such as PALM, STORM and GSDIM and, in its uncaged form, also performs well in STED microscopy. It performs very well with Leica GSD microscope and other superresolution microscopes such as Nikon N-Storm, particularly as a 2 Color partner with CAGE 552 or FLIP 565.
Further, after photoactivation, Abberior CAGE 500 can be tracked to analyze molecular dynamics such as diffusion, flow directions and velocities (e.g. are tracking experiments in which the fluorescence signal is observed over time after the initial release of the fluorophore).


Photophysical properties (carboxylic acid):
Absorption Maximum, λ_ex [nm]: 511 (photoactivated, PBS pH 7.4), 299 (non-photoactivated, PBS pH 7.4)
Extinction Coefficient, ε_max [M^-1cm^-1]: 50 000 (photoactivated, PBS pH 7.4)
Photoactivation Wavelength, λ [nm]: 360-440
Fluorescence Maximum, λ_em [nm]: 525 (photoactivated, PBS pH 7.4)
Recommended STED Wavelength, λ [nm]: 590-610
Fluorescence Quantum Yield, λ: 0,85 (photoactivated, PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 4,1 (photoactivated, PBS pH 7.4)

Eignung

Designed and tested for fluorescent super-resolution microscopy

Hinweis zur Analyse

May contain significant amounts of BSA as stabilizer
unconjugated dye ≤5% of total fluorescence

Sonstige Hinweise

Find more infomation here Abberior^®

Rechtliche Hinweise

abberior is a registered trademark of Abberior GmbH

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable