16805

Atto 425 NHS-Ester

BioReagent, suitable for fluorescence, ≥90% (HPLC)

• Synonym(e): Atto 425-N-hydroxysuccinimidester
• Empirische Formel (Hill-System): C₂₆H₃₀N₂O₈
• CAS-Nummer: 892156-28-4
• Molekulargewicht: 498.53
• NACRES: NA.32
• PubChem Substance ID: 57647590
• UNSPSC Code: 12352108
• MDL number: MFCD04974819

Eigenschaften

• InChI: 1S/C26H30N2O8/c1-5-34-24(32)18-12-16-11-17-15(2)14-26(3,4)27(19(17)13-20(16)35-25(18)33)10-6-7-23(31)36-28-21(29)8-9-22(28)30/h11-13,15H,5-10,14H2,1-4H3
• SMILES string: CCOC(=O)C1=Cc2cc3C(C)CC(C)(C)N(CCCC(=O)ON4C(=O)CCC4=O)c3cc2OC1=O
• InChI key: PUEQEMDTFPYCDY-UHFFFAOYSA-N
• product line: BioReagent
• assay: ≥90% (HPLC), ≥90% (degree of coupling)
• manufacturer/tradename: ATTO-TEC GmbH
• solubility: DMF: soluble, DMSO: soluble, H₂O: soluble
• λ: in ethanol (with 0.1% trifluoroacetic acid)
• UV absorption: λ: 422-428 nm A_max
• suitability: suitable for fluorescence
• storage temp.: −20°C
• Quality Level: 100

Beschreibung

General description

Atto 425 is a member of the commercially available coumarin derivatives.[1]

Application

Die Atto Fluoreszenzfarbstoffe wurden für die hochsensitive Anwendung bis hin zur Einzel-Molekül-Detektion (SMD) entwickelt. Sie haben eine starre Molekülstruktur, was das Auftreten einer cis-trans-Isomerisierung unterbindet. Daher besitzen diese Farbstoffe eine ausserordentlich hohe Fluoreszenzintensität mit einer geringen spektralen Verschiebung nach der Kopplung an Biomoleküle.

Atto 425 NHS ester is used in the development of bifunctional fluorescent, inorganic micro-partices (Zeolite L crystals) designed for targeting or imaging mutliple components . Reacts with amino groups or amine modified oliognucleotides to form a stable amine bond. Utilized as an essential component of molecular beacons, which were designed for use in real-time PCR assays to allow for rapid and accurate identification of gram-negative bacteria . When conjugated with benzylguanine, the fluorescence of Atto 425-NHS is quenched but becomes fluorescent upon labeling of the SNAP-tag fusion protein, making this conjugate an ideal candidate for experiments in living cells . Used as one of the donor probes in three-color FRET experiments designed to investigate the kinetics of RNA-protein complex formation .

Other Notes

Stabilitätsstudien für die Anregung mit einem und zwei Photonen in Fluoreszenz-Korrelations-Spektroskopie[2]; Benutzt für die Dreifarben-Analyse[3]

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)