ST1711

Anti-DFHR Mouse mAb (2B10)

liquid, clone 2B10, Calbiochem^®

• Synonym(e): Anti-Dihydrofolate Reductase
• UNSPSC-Code: 12352203

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: 2B10, monoclonal
• Form: liquid
• Enthält nicht: preservative
• Speziesreaktivität: rat, human
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze; avoid repeated freeze/thaw cycles
• Isotyp: IgG2a
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... DHFR(1719)

Beschreibung

Allgemeine Beschreibung

Purified mouse monoclonal antibody. Recognizes the ~20-22 kDa DHFR protein.

Recognizes the ~20-22 kDa DHFR protein in HeLa and PC-12 cells.

This Anti-DFHR Mouse mAb (2B10) is validated for use in ELISA, Immunoblotting, Immunocytochemistry for the detection of DFHR.

Immunogen

A recombinant polypeptide corresponding to amino acids of 88-188 human DHFR, expressed as a GST fusion protein

Anwendung

ELISA (see comments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (10 g/ml)

Warnhinweis

Toxicity: Regulatory Review (Z)

Physikalische Form

In PBS, pH 7.2.

Rekonstituierung

Following initial thaw, aliquot and freeze (-20°C).

Hinweis zur Analyse

Negative Control
293T cells

Positive Control
HeLa cells, PC-12 Cells

Sonstige Hinweise

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Vodenicharov, M.D., et al. 2010. The EMBO Journal29, 3007.
Ionova, I.A., et al. 2008. Am. J. Physiol. Heart Circ. Physiol.295 H2178.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: nwg
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable