The specific retention time of this dye has not been determined. However, the intracellular retention and fluorescent properties may be comparatively more stable when compared to fluorescein due to the nitro BODIPY-based fluorescent moiety present in SCT035.
SCT035
BioTracker 515 Green ONOO- Dye
Live cell imaging dye for peroxynitrite (ONOO-) used to detect reactive oxygen species (ROS) generated from the interaction of nitric oxide (NO) and superoxide (O).
Synonym(e):
Live cell imaging probe
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Über diesen Artikel
UNSPSC Code:
12352207
NACRES:
NA.47
eCl@ss:
32161000
Preise und Verfügbarkeit sind derzeit nicht verfügbar.
Technischer Dienst
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Unterstützung erhaltentechnique(s)
cell based assay: suitable
detection method
fluorometric
shipped in
ambient
storage temp.
-10 to -25°C
General description
Peroxynitrite (ONOO-) is a strong oxidant generated from the interaction of nitric oxide (NO) and superoxide (O). The in vivo formation of this compound has recently been demonstrated in macrophages, neutrophils and endothelial cells. The role of ONOO- in inflammatory diseases is further supported by the detection of immunoreactive nitrotyrosine on proteins at sites of inflammation.
The BioTracker 515 Green ONOO- Dye is a fluorescent probe that detects peroxynitrite (ONOO-) in living cells. Fluorescent intensity is not increased by the existence of hydroxyl radical, singlet oxygen, hydrogen peroxide, hypochlorite, nitric oxide or superoxide.
Spectral Properties
Absorbance: 490nm
Emission: 515nm
The BioTracker 515 Green ONOO- Dye is a fluorescent probe that detects peroxynitrite (ONOO-) in living cells. Fluorescent intensity is not increased by the existence of hydroxyl radical, singlet oxygen, hydrogen peroxide, hypochlorite, nitric oxide or superoxide.
Spectral Properties
Absorbance: 490nm
Emission: 515nm
Preparation Note
Store BioTracker 515 Green ONOO- Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
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Dieser Artikel | |||
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| description Live cell imaging dye for peroxynitrite (ONOO-) used to detect reactive oxygen species (ROS) generated from the interaction of nitric oxide (NO) and superoxide (O). | description Live cell imaging dye for zinc (Zn2+) for cell signaling, protein self-assembly and cellular metabolism applications. | description Live cell imaging dye for zinc (Zn2+) for cell signaling, protein self-assembly and cellular metabolism applications. | description Live cell imaging dye for hypoxia that increases in fluorescence intensity with decreasing oxygen levels. |
| technique(s) cell based assay: suitable | technique(s) cell based assay: suitable | technique(s) cell based assay: suitable | technique(s) cell based assay: suitable |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
| storage temp. -10 to -25°C | storage temp. -10 to -25°C | storage temp. -10 to -25°C | storage temp. -10 to -25°C |
| detection method fluorometric | detection method fluorometric | detection method fluorometric | detection method fluorometric |
Lagerklasse
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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How long is the dye stable and how long can I monitor the dye under the microscope?
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