PC05

Anti-c-Fos (Ab-2) (4-17) Rabbit pAb

liquid, Calbiochem^®

• NACRES: NA.41
• UNSPSC Code: 12352203
• Clone: polyclonal
• Species reactivity: human, mouse, rat
• Application: —
• Citations: 42

Eigenschaften

• biological source: rabbit
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• species reactivity: human, mouse, rat
• manufacturer/tradename: Calbiochem^®
• storage condition: do not freeze
• isotype: IgG
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... FOS(2353)

Beschreibung

General description

This Anti-c-Fos (Ab-2) (4-17) Rabbit pAb is validated for use in Frozen sections, Immunoblotting, IF, IP, Paraffin sections, for the detection of c-Fos (Ab-2) (4-17).

Protein A purified rabbit polyclonal antibody. Recognizes v-fos and ~50-62 kDa c-fos proteins.

Recognizes the ~50-62 kDa c-Fos protein in HepG2, NIH3T3, and MCF-7 cells.

The proto-oncogene fos has been implicated in cell growth, differentiation and development. Fos is induced by a large number of stimuli including mitogens, differentiation-specific agents, pharmacological agents, etc. Induction of the 62,000 dalton fos protein is rapid but transient. The fos protein is associated with a 39,000 dalton protein which has been identified as the protein product of the jun proto-oncogene (c-jun). The fos/jun protein complex binds specifically to a sequence element in DNA referred to as the AP-1 binding site.

Immunogen

Human

a synthetic peptide (SGFNADYEASSSRC) (Cat. No. PP10) corresponding to amino acids 4-17 of human c-Fos

Application

Frozen sections (5-10 µg/ml)

Immunoblotting (1-5 µg/ml)

Immunofluorescence (1-5 µg/ml)

Immunoprecipitation (1-2 µg/reaction)

Paraffin sections (5-10 µg/ml, pepsin or heat pre-treatment required)

Free-floating Sections (not recommended)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 0.05 M sodium phosphate buffer, 0.2% gelatin.

Analysis Note

Positive Control
HepG2, NIH 3T3 cells, MCF-7 cells, colon or breast adenocarcinoma tissue

Other Notes

DeTogni, P., et al. 1988. Cell Biol.8, 2251.
Rouscher III, F.J., et al. 1988. Science240, 1010.
Sassone-Corsi, P., et al. 1988. Cell54, 553.
Verma, I.M. and Graham, W.R. 1987. Cancer Res.49, 29.
Verma, I.M. and Sassone-Corsi, P. 1987. Cell51, 513.
Muller, R. 1986. Biochim. Biophys. Acta823, 207.
Verma, I. 1986. Trends Genet.2, 93.
Greenberg, M. and Ziff, E. 1984. Nature (London), 311, 433.

For gel shift assays, use Cat. No. PC05L resuspended in 100 µl of buffer. The immunogen, c-fos (Peptide-2), Cat. No. PP10, is also available for competition studies (see Murphy, et al. below). Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Highly Toxic (H)

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: nwg