MABE2184

Anti-Islet-1 homeobox Antibody, clone 39.3F7

• Synonym(e): Insulin gene enhancer protein ISL-1
• NACRES: NA.43
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: 39.3F7, monoclonal
• Application: EM, FACS, IF, IHC, IP, WB
• Citations: -

Eigenschaften

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 39.3F7, monoclonal
• mol wt: calculated mol wt 39.04 kDa, observed mol wt ~35 kDa
• purified by: using protein G
• species reactivity: rat, mouse, human, chicken
• packaging: antibody small pack of 100
• technique(s): electron microscopy: suitable, flow cytometry: suitable, immunofluorescence: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG1κ
• epitope sequence: C-terminal
• Protein ID accession no.: NP_059035
• UniProt accession no.: P61374
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: rat ... Isl1(64444)

Beschreibung

General description

Islet-1 homeobox (UniProt: P61374) also known as Islet-1, is encoded by the Isl1 gene (Gene ID: 64444) in rat. Islet-1 homeobox protein is a transcription factor that plays a crucial role in the development of various tissues, including the heart, motor neurons, and sensory neurons. It is a member of the LIM-homeodomain transcription factor family and is involved in the regulation of gene expression during embryonic development. ISL1 is particularly significant in the development of the heart, where it contributes to the formation of the cardiac outflow tract and the differentiation of pacemaker and conduction cells. In addition to its role in cardiac development, ISL1 is also involved in the specification and differentiation of motor neurons and sensory neurons. Its expression is tightly regulated, and mutations in the ISL1 gene have been associated with congenital heart defects and developmental disorders.

Immunogen

Recombinant fusion protein corresponding to 172 amino acids from the C-terminal part of rat Islet-1 homeobox protein.

Application

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Islet-1 homeobox in HeLa cell lysate.

Tested Applications

Flow Cytometry Analysis: A representative lot detected Islet-1 homeobox in Flow Cytometry applications (Foo, K.S., et al. (2018). Mol Ther. 26(7):1644-1659).

Immunohistochemistry Analysis: A representative lot detected Islet-1 homeobox in Immunohistochemistry applications (Chai, G., et al. (2014). Nat. Neurosci. 17(9):1171-9; Smith, R., et al. (2017). J. Neurosci. 37(33):7975-7993; Schick, E., et al. (2019). Sci Rep. 9(1):9358).

Western Blotting Analysis: A representative lot detected Islet-1 homeobox in Western Blotting applications (Foo, K.S., et al. (2018). Mol. Ther. 26(7):1644-1659).

Immunoprecipitation Analysis: A representative lot immunoprecipitated Islet-1 homeobox in Immunoprecipitation applications (Velasco, S., et al. (2017). Cell Stem Cell. 20(2):205-217).

Immunofluorescence Analysis: A representative lot detected Islet-1 homeobox in Immunofluorescence applications (Smith, R., et al. (2017). J. Neurosci. 37(33):7975-7993; Foo, K.S., et al. (2018). Mol. Ther. 26(7):1644-1659; Schick, E., et al. (2019). Sci. Rep. 9(1):9358).

Electrophoretic Mobility Shift Assay: A representative lot detected Islet-1 homeobox in Electrophoretic Mobility Shift Assay applications (Du, A., et al. (2009). Diabetes. 58(9):2059-69).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Biochem/physiol Actions

Clone 39.3F7 is a mouse monoclonal antibody that detects Islet-1 homeobox. It targets an epitope within 172 amino acids from the C-terminal part of the protein.

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable