MABE1025

Anti-Tankyrase-2 Antibody, clone 2F5.1

clone 2F5.1, from mouse

• Synonym(e): TANK2, Tankyrase-2, ADP-ribosyltransferase diphtheria toxin-like 6, ARTD6, Poly [ADP-ribose] polymerase 5B, TNKS-2, TRF1-interacting ankyrin-related ADP-ribose polymerase 2, Tankyrase II, Tankyrase-like protein, Tankyrase-related protein
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: 2F5.1, monoclonal
• Speziesreaktivität: human
• Methode(n): western blot: suitable
• Isotyp: IgG2bκ
• NCBI-Hinterlegungsnummer: NP_079511
• UniProt-Hinterlegungsnummer: Q9H2K2
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... TNKS2(80351)

Beschreibung

Allgemeine Beschreibung

Tankyrase-2 (EC 2.4.2.30; UniProt Q9H2K2; also known as ADP-ribosyltransferase diphtheria toxin-like 6, ARTD6, PARP-5B, Poly [ADP-ribose] polymerase 5B, TANK2, TNKS-2, Tankyrase II, Tankyrase-like protein, Tankyrase-related protein, TRF1-interacting ankyrin-related ADP-ribose polymerase 2) is encoded by the TNKS2 (also known as PARP5B, TANK2, TNKL) gene (Gene ID 80351) in human. Tankyrase-2 belongs to the poly(ADP-ribosylation) polymerase (PARP) family of enzymes that mediate post-translational modification of proteins by poly(ADP-ribosyl)ation, where repeating units of ADP-ribose linked by glycosidic bonds were added to aspartate, glutamate, asparagine, arginine or lysine residues of substrate proteins. Poly(ADP-ribose) (PAR) modification is reversible through the action of poly(ADP-ribose) glycohydrolase (PARG) and ADP-ribosyl protein lyase. Tankyrase-2 and the related Tankyrase-1 share a common domain organization with 15 N-terminal ankyrin-repeats (a.a. 57-776 in TNKS2) involved in substrate recognition, a sterile alpha motif (SAM) domain (a.a. 873-936 in TNKS2) required for dimerization, followed by the C-terminal PARP domain (a.a. 959-1164 in TNKS2). Mice with either Tnks1- or Tnks2-knockout are viable, while double Tnks1-/Tnks2-knockout is lethal at embryonic day 9.5, suggesting a functional redundancy between these two enzymes. Both tankyrases target and suppress Axin2, a well known β-catenin negative regulator. Tankyrase-2 ribosylates Src-binding adapter protein 3BP2, leading to its ubiquitination and proteasomal degradation in macrophages and osteoclasts. Loss of tankyrase-mediated 3BP2 negative regulation underlies the pathogenic mechanism of cherubism, an autosomal dominant disorder affecting cranial bone development.

Spezifität

Clone 2F5.1 recognizes an epitope in the region between ANK repeats 6 & 8 of human Tanyrase-2.

Immunogen

Epitope: Region between ANK repeats 6 & 8.

GST-tagged recombinant human Tankyrase-2 fragment from the region between ANK repeats 6 & 8.

Anwendung

Research Category
Epigenetik & nukleäre Funktionen

Research Sub Category
Allgemeine Posttranslationsmodifikation

Anti-Tankyrase-2 Antibody, clone 2F5.1 is an antibody against Tankyrase-2 for use in Western Blotting.

Qualität

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Tankyrase-2 in 10 µg of Jurkat cell lysate.

Zielbeschreibung

~126 kDa observed. 126.9 kDa (calculated). Uncharacterized band(s) may appear in some lysates.

Physikalische Form

Protein G purified.

Format: Purified

Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable