MAB3890

Anti-PPAR α Antibody

ascites fluid, Chemicon^®

• Synonym(e): PPAR alpha
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: monoclonal
• Application: ELISA, ICC, WB
• Citations: 10

Eigenschaften

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: monoclonal
• species reactivity: human, mouse
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable, immunocytochemistry: suitable, western blot: suitable
• isotype: IgG2a
• NCBI accession no.: NM_001001928.2, NM_001001929.2, NM_001001930.2, NM_005036.4, NM_032644.3
• UniProt accession no.: Q07869
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... PPARA(5465)

Beschreibung

General description

Peroxisome proliferator-activated receptors (PPARs) are nuclear hormone receptors that can be activated by a variety of compounds including fibratus, thiazolidinediones, prostaglandins and fatty acids. Three PPAR subtypes, designated PPARα, PPARβ (also designated PPARδ) and PPARγ, have been described. PPARs promote transcription by forming heterodimers with members of the retinoid X receptor (RXR) family of steroid receptors and binding to specific DNA motifs termed PPAR-response elements (PPREs). PPARα is abundant in primary hepatocytes where it regulates the expression of proteins involved in fatty acid metabolism. PPARβ is the most widely distributed subtype and is often expressed at high levels. PPARγ is predominantly seen in adipose tissue where it plays a critical role in regulating adipocyte differentiation.

Immunogen

Synthetic peptide from amino acids 18-34 of mouse PPARalpha.

Application

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Use Anti-PPAR α Antibody (Mouse Monoclonal Antibody) validated in ELISA, WB, ICC to detect PPAR alpha also known as Peroxisome Proliferator Activated Receptor α.

Western blot: 1:500-1:5,000

Immunocytochemistry: 1:500-1:5,000

ELISA: 1:500-1:5,000

Optimal working dilutions must be determined by end user.

Biochem/physiol Actions

Reacts with Peroxisome Proliferator Activated Receptor alpha (PPARalpha). No cross reactivity with PPARbeta or PPARgamma.

Physical form

Ascites fluid. Liquid. Contains no preservative.

Preparation Note

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Analysis Note

Control
3T3 whole cell lysate

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable