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PhosphoDetect Anti-ATM (pSer¹⁹⁸¹) Mouse mAb (10H11.E12)

liquid, clone 10H11.E12, Calbiochem®

Synonym(e):

Anti-Ataxia Telangiectasia, Phospho-Specific (Ser¹⁹⁸¹)

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About This Item

UNSPSC-Code:
12352203

Biologische Quelle

mouse

Qualitätsniveau

Antikörper-Produkttyp

primary antibodies

Klon

10H11.E12, monoclonal

Form

liquid

Enthält

≤0.1% sodium azide as preservative

Speziesreaktivität

human, mouse

Hersteller/Markenname

Calbiochem®

Lagerbedingungen

OK to freeze
avoid repeated freeze/thaw cycles

Isotyp

IgG1

Versandbedingung

wet ice

Lagertemp.

−20°C

Allgemeine Beschreibung

Protein G purified mouse monoclonal antibody. Recognizes the ~370 kDa ATM protein phosphorylated at Ser1981.
Recognizes the ~370 kDa ATM protein phosphorylated on Ser1981 in camptothecin-treated HeLa cells. Sold under license of U.S. Patent 7,108,992.
This PhosphoDetect Anti-ATM (pSer¹⁹⁸¹) Mouse mAb (10H11.E12) is validated for use in Immunoblotting, Immunocytochemistry for the detection of ATM (pSer¹⁹⁸¹).

Immunogen

Human
a synthetic phosphopeptide (SLAFEEGpSQSTTISS) corresponding to amino acids 1974-1988 surrounding the Ser¹⁹⁸¹ phosphorylation site of human ATM

Anwendung





Immunoblotting (0.5 g/ml)
Immunocytochemistry (1.5 g/ml)

Verpackung

Please refer to vial label for lot-specific concentration.

Warnhinweis

Toxicity: Irritant (B)

Physikalische Form

In PBS.

Rekonstituierung

Following initial thaw, aliquot and freeze (-20°C).

Hinweis zur Analyse

Positive Control
HeLa cells treated with camptothecin (Cat. No. 208925)

Sonstige Hinweise

Does not detect the unphosphorylated protein. Use 5% milk in TBS-T for blocking; the use of BSA is not recommended. The use of PBS-T is not recommended, as phosphate-containing buffers can contribute to high background with phosphospecific antibodies. Antibody should be titrated for optimal results in individual systems.
Horejsi, Z., et al. 2004. Oncogene23, 3122.
Abraham, R.T. 2003. Genes Dev.15, 2177.
Bakkenist, C.J. and Kastan, M.B. 2003. Nature421, 499.
Shiloh, Y. 2003. Nat. Rev. Cancer3, 155.

Rechtliche Hinweise

Sold under license of U.S. Patent 7,108,992.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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