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ACK5010NT

Amicon® Pro Affinity Concentration Kit Ni-NTA with 10kDa Amicon® Ultra-0.5 Device

Amicon Pro Affinity Concentration Kit Ni-NTA - A Centrifugal Tool for purifying & concentrating His-tagged proteins.

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eCl@ss:
36100101
UNSPSC Code:
41104916

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material

Amicon Ultra styrene-butadiene device, Ultracel® regenerated cellulose membrane (Amicon Ultra-0.5; low binding), polyethylene cap, polyethylene frit, polypropylene device (for exchange), polypropylene frit, polypropylene tube (AU0.5; for collection), polypropylene tube (for collection), polypropylene tube (holder)

sterility

non-sterile

product line

Amicon®

manufacturer/tradename

Amicon® Pro

parameter

10 mL sample volume

technique(s)

buffer exchange: suitable, protein purification: suitable (antibody labeling), protein purification: suitable (concentration), protein purification: suitable (enrichment)

L

12 cm (4.7 in.)

diam.

2.97 cm

filtration area

1 cm2

pore size

10 kDa NMWCO

shipped in

wet ice

General description

Filter Code: PLCGC
Filter Type: Ultrafiltration
This kit includes 12/pk Amicon Pro, 12/pk Amicon Ultra NMWCO 10 kDa, 3 ml HisBind Ni-NTA resin (Binding capacity: 5 to 10mg His tagged protein/ml agarose), 25ml Bind Buffer (4X), 25 ml Wash buffer (4X), 5ml elution buffer (4X)

Application

Amicon Pro Affinity Concentration Kit Ni-NTA - A Centrifugal Tool for purifying & concentrating His-tagged proteins.

Features and Benefits

Minimum Final Concentrate Volume: 15 µL

Physical form

Color Code: Clear with pink cap

Legal Information

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

signalword

Danger

Hazard Classifications

Eye Dam. 1 - Flam. Liq. 3 - Repr. 1B - Skin Corr. 1C

Lagerklasse

3 - Flammable liquids

flash_point_f

95.9 °F

flash_point_c

35.5 °C

wgk

WGK 2

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Chiara Orlandi et al.
Structure (London, England : 1993), 28(5), 516-527 (2020-03-27)
Antibody structure couples adaptive and innate immunity via Fab (antigen binding) and Fc (effector) domains that are connected by unique hinge regions. Because antibodies harbor two or more Fab domains, they are capable of crosslinking multi-determinant antigens, which is required

Verwandter Inhalt

Amicon®Pro Purification System - Choose the direct route. Purify with a pro.

bios-C-epdf-1043-1304-SP

Purify biologically active proteins with gentle, all-in-one recovery: Amicon Pro purification system.

When you start with consistent yields of active, native-folded protein, you’re giving your experiment the best chance to succeed. If your current protein preps involve juggling columns, dialyzers and multiple transfer steps, you could be introducing variability into your data. For your next protein preparation, choose the simple, gentle method that tackles even the most labile and poorly expressed proteins—the Amicon® Pro purification system. When your proteins behave, your research will flourish.

Amicon® Pro Affinity Concentration Kit - Ni-NTA
Amicon®Pro Purification System

Two attributes of the Amicon® Pro device make it a convenient device for preparing pure, labeled antibody. First, the device enables highly efficient buffer exchange via diafiltration with simultaneous sample concentration in a single 15 minute spin. Second, the entire workflow can be performed within a single device, reducing the potential for sample loss. In this report, we describe the successful use of the Amicon® Pro device for small–scale biotinylation of a target antibody. The biotinylated antibody was used for detection of protein immunoprecipitated from cell lysate.

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