ABE2882

Anti-Histone H3 Antibody, K14M mutant

• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: polyclonal
• Technique(s): dot blot: suitable, western blot: suitable
• Application: DB, WB
• Citations: -

Eigenschaften

• antibody form: purified antibody
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: rabbit, human
• concentration: (Please refer to lot specific datasheet.)
• technique(s): dot blot: suitable, western blot: suitable
• isotype: IgG
• UniProt accession no.: P68431

Beschreibung

General description

KLH-conjugated linear peptide corresponding to 11 amino acids from the N-terminal region of Human Histone H3 with lysine 14 substituted with methionine.

~19 kDa observed; 15.40 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Application

Epitope: N-terminus

Biochem/physiol Actions

This rabbit polyclonal antibody specifically detects Histone H3 with K14M substitution.

Physical form

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Evaluated Anti-Histone H3, K14M mutant by Dot Blot using H3.1 peptide.



Dot Blot Analysis: 2.5 to 20 ng of this antibody detected Histone H3.1 peptide containing the K14M substitution.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.