AB9674

Anti-Tau phospho Serine 199/202 Antibody

Chemicon^®, from rabbit

• Synonym(e): Anti-Anti-DDPAC, Anti-Anti-FTDP-17, Anti-Anti-MAPTL, Anti-Anti-MSTD, Anti-Anti-MTBT1, Anti-Anti-MTBT2, Anti-Anti-PPND, Anti-Anti-PPP1R103, Anti-Anti-TAU, Anti-Anti-tau-40
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: polyclonal
• Species reactivity: human
• Application: WB
• Citations: 17

Eigenschaften

• biological source: rabbit
• Quality Segment: 100
• antibody form: affinity purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• manufacturer/tradename: Chemicon^®
• technique(s): western blot: suitable
• NCBI accession no.: NM_005910.3, NM_016834.2, NM_016835.2, NM_016841.2
• UniProt accession no.: P10636
• shipped in: dry ice
• target post-translational modification: phosphorylation (pSer199/pSer202)
• Gene Information: human ... MAPT(4137)

Beschreibung

General description

Tau is a neuronal microtubule-associated protein found predominantly on axons and functions to promote tubulin polymerization and stabilize microtubules. Tau, in its hyperphosphorylated form, is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer′s disease (AD) brain. Hyperphosphorylated Tau is also found in neurofibrillary lesions in a range of other central nervous system disorders. Hyperphosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases, including GSK-3beta, protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II (CK2), phosphorylate Tau. Serines 199 and 202 are phosphorylated by GSK-3beta and have been linked to hereditary frontotemporal dementia. Serine 202 phosphorylation by cdk5, stimulated by the presence of microtubules, has been linked to hereditary neurodegenerative disease.

Immunogen

Synthetic peptide of amino acids surrounding the phosphoSerine 199 and 202 sites of human Tau.

Application

Detect Tau phospho Serine 199/202 using this Anti-Tau phospho Serine 199/202 Antibody validated for use in WB.

Research Category
Neuroscience

Research Sub Category
Neurodegenerative Diseases

Western blot: 1:1,000. Suggested blocking buffer is 5% BSA-TBST overnight at 2-8°C. Suggested antibody dilution buffer is 3% BSA-TBST. Suggested antibody incubation time is 2 hours at room temperature.

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Tau phosphoSerine 199/202. The antibody recognizes Tau pSerine 199/202 in samples of recombinant human Tau treated with GSK-3beta for 45 minutes. The reactivity of the antibody is blocked with the pSerine 199/202 peptide but not the non-phosphopeptide, tau phosphopeptide corresponding to pSerine 199, tau phosphopeptide corresponding to pSerine 202 or a generic phosphoSerine-containing peptide.

Preparation Note

Maintain at -20°C in undiluted for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self defrosting freezer.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 2