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70751

BugBuster® Ni-NTA His•Bind® Purification Kit

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NACRES:
NA.56
UNSPSC Code:
41106500
Shipped in:
wet ice

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manufacturer/tradename

Novagen®

storage condition

OK to freeze

shipped in

wet ice

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Dieser Artikel
70666GE28-9034-6670691
BugBuster® Ni-NTA His•Bind® Purification Kit

Millipore

70751

BugBuster® Ni-NTA His•Bind® Purification Kit

Ni-NTA His•Bind® Resin

Millipore

70666

Ni-NTA His•Bind® Resin

illustra™ GFX PCR DNA and Gel Band Purification Kit, 10 purifications

GE28-9034-66

illustra™ GFX PCR DNA and Gel Band Purification Kit, 10 purifications

Ni-NTA His•Bind® Superflow™ Resin

Millipore

70691

Ni-NTA His•Bind® Superflow™ Resin

manufacturer/tradename

Novagen®

manufacturer/tradename

Novagen®

manufacturer/tradename

Cytiva

manufacturer/tradename

Novagen®

shipped in

wet ice

shipped in

ambient

shipped in

-

shipped in

ambient

storage condition

OK to freeze

storage condition

do not freeze

storage condition

-

storage condition

do not freeze

General description

BugBuster®Ni-NTA HIS-BIND® Purification Kit is used for protein purification. Ni-NTA HIS-BIND® Resin is high-performance Ni2+-charged agarose used for rapid, one-step purification of proteins containing a polyhistidine tag sequence.

Application

BugBuster®Ni-NTA HIS-BIND® Purification Kit has been used for the purification of His tagged proteins such as trehalose-6-phosphate phosphatase (TPP) [1], Ras(WT) proteins[2] and CsgA (major curlin subunit) proteins.[3]

Other Notes

•2 × 100 mlBugBuster Protein Extraction Reagent

•10,000 UBenzonase Nuclease, purity >90%

•10 mlNi-NTA His•Bind Resin

•pkg/4Chromatography Columns
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Legal Information

BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS-BIND is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Lagerklasse

3 - Flammable liquids

wgk

WGK 3

Hazard Classifications

Flam. Liq. 3

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Chae-Seok Lim et al.
Small (Weinheim an der Bergstrasse, Germany), 13(40) (2017-08-16)
Intermolecular interactions dominate the behavior of signal transduction in various physiological and pathological cell processes, yet assessing these interactions remains a challenging task. Here, this study reports a single-molecule force spectroscopic method that enables functional delineation of two interaction sites
Sagar Lahiri et al.
Journal of cellular physiology, 229(9), 1245-1255 (2014-01-22)
Trehalose-6-phosphate phosphatase (TPP) catalyzes the final step in the biosynthesis of the anti-stress sugar trehalose. An 82 kDa TPP enzyme was isolated from Candida utilis with 61% yield and 43-fold purification. The protein sequence, determined by N-terminal sequencing and MALDI-TOF analysis
Sarah A Tursi et al.
PLoS pathogens, 13(4), e1006315-e1006315 (2017-04-15)
Bacterial biofilms are associated with numerous human infections. The predominant protein expressed in enteric biofilms is the amyloid curli, which forms highly immunogenic complexes with DNA. Infection with curli-expressing bacteria or systemic exposure to purified curli-DNA complexes triggers autoimmunity via

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BugBuster® and Benzonase® Reagents are the Clear Solutions to Simple, Efficient Extraction of E. coli Proteins

This article shows the use of BugBuster® and Benzonase® as protein purification tools to extract recombinant proteins from E. coli and to reduce the viscosity of the extract.

Verwandter Inhalt

New, combined lysis and purification reaction simplifies recombinant protein purification with magnetic beads

Traditionally, protein purification from E. coli consists of four distinct phases: harvest, bacterial cell lysis, lysate clarification and protein purification. Bacterial lysis typically requires several time-consuming, hands-on steps, such as freeze/thaw cycles and sonication. These harsh lysis techniques may negatively impact protein quality and contribute to sample-to-sample variability. To maintain protein activity and integrity, detergent-based lysis buffers are routinely used to avoid mechanical protein extraction methods. Regardless of the lysis method used, centrifugation is traditionally required to pellet unwanted cell debris and permit recovery of the clarified lysate. The final step, purification, is frequently performed using affinity media specific for expressed epitope tags. Agarose-based media have typically been used, either as a slurry in microcentrifuge tubes or packed into gravity-driven or spin columns. While easier to manipulate, columns are greatly affected by lysate consistency and carryover of cell debris, which can lead to clogging of the column frits.

Protein Purification and Detection Tools
Ni-NTA HisﾕBind® Resins
安定したタンパク質抽出と効率的なサンプル調整に　Buster & Protein Extraction Kit シリーズとタンパク質調整関連製品

Global Trade Item Number

SKUGTIN
369071-5G04061837008450
70751-304055977273168

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