70239-M

His•Bind^®-Aufreinigungskit

• Synonym(e): IMAC, Protein purification
• NACRES: NA.56
• UNSPSC Code: 41106500

Eigenschaften

• manufacturer/tradename: Novagen^®
• Quality Segment: 400
• storage condition: OK to freeze
• storage temp.: 2-8°C

Beschreibung

General description

HIS-BIND^® purification kit includes pre-tested buffers specifically designed for IDA-based HIS-BIND Resin, enabling convenient and rapid one-step purification of proteins by metal affinity chromatography. It uses the HIS TAG^®/HIS-BIND^® technology, that leverages the affinity between neighboring histidine in the HIS TAG sequence and immobilized metal ions (such as Ni²⁺ or Co²⁺).

Application

HIS-BIND^® Purification Kit has been used for: the purification of N-terminal 6-histidine-tagged aldehyde dehydrogenase isozyme, ALDH1A1 under nondenaturing conditions by nickel-NTA affinity chromatography, the purification of virus binding protein specific for Poliovirus Type 1, the purification of recombinant oE7, huhsp70 and oE7/huhsp70 proteins, to study the effective antitumor efficacy of human hsp70 and HPV16 oE7 fusion protein vaccine, the purification of recombinant exFPR1, MTBHsp70 and MTBHsp70-exFPR1 fusion proteins to study their role in cervical cancer immunotherapy

Features and Benefits

• Includes HIS-BIND^® resin, HIS-BIND^® buffer kit and chromatography columns.
• Solutions are included for Ni²⁺ charging, binding, washing, and elution.
• Columns are pre-packed with 1.25 ml bed volume of Ni²⁺-charged HIS-BIND resin.

Other Notes

•10 mlHis•Bind-Harz

•1His•Bind-Puffer-Kit

•Pack./4Chromatographiesäulen

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Legal Information

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS-BIND is a registered trademark of Merck KGaA, Darmstadt, Germany

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxizität: Mehrere Toxizitätswerte, siehe MSDS (O)

Sicherheitshinweise

• pictograms: GHS02,GHS08,GHS05,GHS09
• signalword: Danger
• hcodes: H226,H314,H317,H334,H341,H350i,H360D,H372,H373,H411
• pcodes: P210 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Aquatic Chronic 2 - Carc. 1A Inhalation - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Corr. 1C - Skin Sens. 1 - STOT RE 1 - STOT RE 2 Inhalation
• Lagerklasse: 3 - Flammable liquids
• flash_point_f: 86.0 °F
• flash_point_c: 30 °C
• wgk: WGK 3
• target_organs: respiratory tract irritation, respiratory tract irritation,Respiratory Tract