69522

T7•Tag^® Monoclonal Antibody

• Synonym(e): T7 Tag Antibody
• UNSPSC-Code: 12352203

Eigenschaften

• Klon: monoclonal
• Speziesreaktivität: mouse
• Hersteller/Markenname: Novagen^®
• Lagerbedingungen: OK to freeze; avoid repeated freeze/thaw cycles
• Isotyp: IgG2b
• Versandbedingung: dry ice; wet ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified

Beschreibung

Allgemeine Beschreibung

The T7•Tag^® Antibody is a mouse monoclonal antibody (IgG2b) directed against the 11 amino acid gene 10 leader peptide expressed by many of the pET translation vectors as well as pSCREEN^® and pRSET vectors. Because the peptide is the natural amino terminal end of the T7 major capsid protein, the antibody also recognizes T7 bacteriophage (e.g., the T7Select^® Vectors). For increased versatility and sensitivity, this antibody is also available labeled with biotin and as a direct conjugate with alkaline phosphatase or horseradish peroxidase.

Spezifität

T7•Tag peptide MetAlaSerMetThrGlyGlyGlnGlnMetGly; N-terminal, C-terminal or internal

Cross-reactivity: Negligible with bacterial, insect or mammalian cell lysates

Sensitivity: 1–10 ng (Western blot developed with chromogenic substrates) < 1 ng (AP or HRP conjugate developed with chemiluminescent substrates, or Biotin conjugate developed with Streptavidin AP and chromogenic substrates). Also suitable for immunoprecipitation and immunohistochemistry (1–3)

Working dilution- 1:5,000–1:10,000

Verpackung

Plastic ampoule

Warnhinweis

Multiple Toxicity Values, refer to MSDS

Physikalische Form

Stabilized solution of antibody in 50% glycerol; includes Positive Control Extract containing 31.1 kDa target protein

Sonstige Hinweise

1. Krek, W., et al. 1994. Cell 78,, 161.
2. Roberts, S.G.E. and Green, M. (1994) Nature 371, 717–720.
3. Joneson, T., McDonough, M., Bar-Sag, D., and Van Aelst, L. (1996) Science 274, 1374–1376.

Rechtliche Hinweise

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

PEPscreen is a registered trademark of Sigma-Aldrich Co. LLC

T7-Tag is a registered trademark of Merck KGaA, Darmstadt, Germany

T7SELECT is a registered trademark of Merck KGaA, Darmstadt, Germany