492016

NP-40-Alternative

• Synonym(e): NP-40-Alternative – CAS 9016-45-9 – Calbiochem, Nonylphenylpolyethylenglykol
• CAS-Nummer: 9016-45-9
• UNSPSC Code: 12161902
• NACRES: NA.25
• MDL number: MFCD00132851

Eigenschaften

• description: RTECS - MD0905000
• Quality Segment: 200
• form: liquid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, desiccated
• color: clear colorless
• CMC: 50 - 300 μM
• solubility: water: 25 mg/mL, aqueous buffer: soluble
• density: 1.06 g/mL
• application(s): (Isolating membrane proteins)
• shipped in: ambient
• storage temp.: 15-25°C
• SMILES string: O(CCOc1ccc(cc1)CCCCCCCCC)CCO
• InChI: 1S/C19H32O3/c1-2-3-4-5-6-7-8-9-18-10-12-19(13-11-18)22-17-16-21-15-14-20/h10-13,20H,2-9,14-17H2,1H3
• InChI key: BLXVTZPGEOGTGG-UHFFFAOYSA-N

Beschreibung

General description

Ein nicht-ionisches Tensid, das bei der Isolierung und Reinigung von funktionellen Membranproteinkomplexen und bei der zweidimensionalen (2D-)Gelelektrophorese verwendet wird. Ähnliche Anwendung wie bei NP-40 und TRITON^™ X-100 Detergens (Katalog-Nr. <;A href="https://./?[islink]ViewProductDetail-BySKU&;#124;&;#124;SKU&;#124;EMD_BIO-648462&;#124;[/islink]">;648462<;/A>;).

Application

NP-40 Alternative has been used:

• as a component of the lysis buffer for solubilizing proteins from transfected HEK293 cells.
• as a component of the wash buffer for efficient solubilization of biotinylated plasma proteins in plasma protein profiling and chromatin immunoprecipitation (ChIP).
• for the purification of doublet microtubules from cilia for single particle cryo-electron microscopy (cryo-EM).

Other Notes

Ripoche, P., et al. 1982. Biochim. Biophys. Acta693, 497.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Disclaimer

Toxizität: Reizstoff (B)

Sicherheitshinweise

• pictograms: GHS07,GHS09
• Lagerklasse: 11 - Combustible Solids
• flash_point_f: 503.6 °F - Pensky-Martens closed cup - (own results)
• flash_point_c: 262 °C - Pensky-Martens closed cup - (own results)
• signalword: Danger
• hcodes: H315,H319,H410,EUH430
• pcodes: P201 - P202 - P273 - P391 - P405 - P501
• Hazard Classifications: Aquatic Acute 1 - Aquatic Chronic 1 - ED ENV 1 - Eye Irrit. 2 - Skin Irrit. 2