324903

Epiregulin, Human, Recombinant

Epiregulin, Human, Recombinant, consists of amino acids 63-108 and expressed in E. coli with an N-terminal methionine.

• UNSPSC Code: 12352203

Eigenschaften

• Quality Segment: 100
• assay: ≥97% (SDS-PAGE)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• impurities: <1.0 EU/μg protein Endotoxin
• shipped in: ambient
• storage temp.: −20°C

Beschreibung

General description

Recombinant, human epiregulin consisting of amino acids 63-108 and expressed in E. coli with an N-terminal methionine. Epiregulin, expressed primarily in the placenta and macrophages, stimulates fibroblast, smooth muscle cell, and hepatocyte proliferation.

Biochem/physiol Actions

ED₅₀ = 0.125-0.75 ng/ml as measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblasts.

Physical form

Lyophilized from 0.2 µm sterile-filtered PBS, BSA.

Preparation Note

Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.

Reconstitute to 100 µg/ml in sterile PBS containing at least 0.1% human or bovine serum albumin.

Other Notes

Mishre, R., et al. 2002. Am. J. Physiol. Renal. Physiol.283, F1151.
Shirakata, Y., et al. 2000. J. Biol. Chem.275, 5748.
Shelly, M., et al. 1998. J. Biol. Chem.273, 10496.
Das, S.K., et al. 1997. Dev. Biol.190, 178.
Komurasaki, T., et al. 1997. Oncogene15, 2841.
Toyoda, H., et al. 1997. Biochem. J.326, 69.
Toyoda, H., et al. 1995. J. Biol. Chem.270, 7495.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Sicherheitshinweise

• Lagerklasse: 13 - Non Combustible Solids
• wgk: WGK 3