207008

Calcineurin Substrate

An excellent peptide substrate for calcineurin.

• Synonym(e): Calcineurin Substrate, PP2B Substrate, RII Phosphopeptide, DLDVPIPGRFDRRVpSVAAE
• Empirische Formel (Hill-System): C₉₂H₁₅₁N₂₈O₃₂P
• Molekulargewicht: 2192.33
• NACRES: NA.77
• UNSPSC Code: 12352200
• Assay: ≥95% (HPLC)
• Form: lyophilized
• Storage condition: OK to freeze, desiccated (hygroscopic)

Eigenschaften

• Quality Segment: 100
• assay: ≥95% (HPLC)
• form: lyophilized
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, desiccated (hygroscopic)
• solubility: water: 2 mg/mL
• shipped in: ambient
• storage temp.: −20°C

Beschreibung

General description

An excellent peptide substrate for calcineurin (Protein Phosphatase 2B, PP2B; Cat. No. 539568). The sequence is derived from the PKA regulatory subunit type II (RII) and is phosphorylated on the Ser residue. Release of phosphate can be quantified colorimetrically using the malachite green method.

Biochem/physiol Actions

Cell permeable: no

Primary Target
Calcineurin (Protein Phosphatase 2B, PP2B)

Product does not compete with ATP.

Reversible: no

Physical form

Supplied as a trifluoroacetate salt.

Preparation Note

Following reconstitution, aliquot and freeze at -20°C. Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Donella-Deana, A., et al. 1994. Eur. J. Biochem. 219, 109.
Enz, A., et al. 1994. Anal. Biochem. 216, 147.
Harder, K.W., et al. 1994. Biochem. J. 298, 395.
Fruman, D.A., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 3686.

H-Asp-Leu-Asp-Val-Pro-Ile-Pro-Gly-Arg-Phe-Asp-Arg-Arg-Val-Ser(PO₃H₂)-Val-Ala-Ala-Glu-OH

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable