16-638
4G10® Platinum, Anti-Phosphotyrosine Antibody, Agarose Conjugate
clone 4G10®, Upstate®, from mouse
Synonym(e):
4G10 Platinum Anti-Phosphotyrosine, 4G10 Platinum Antibody, Phosphotyrosine Detection Antibody
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Alle Fotos(1)
About This Item
UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Empfohlene Produkte
Biologische Quelle
mouse
Qualitätsniveau
Antikörper-Produkttyp
primary antibodies
Klon
4G10®, monoclonal
Speziesreaktivität (Voraussage durch Homologie)
all
Hersteller/Markenname
Upstate®
Methode(n)
immunoprecipitation (IP): suitable
Isotyp
IgG2bκ
Versandbedingung
wet ice
Posttranslationale Modifikation Target
phosphorylation (pTyr)
Angaben zum Gen
human ... PID1(55022)
Allgemeine Beschreibung
The development of the anti-phosphotyrosine, clone 4G10 in 1989 was a monumental discovery for researchers. 4G10 was the first and best single monoclonal antibody for the detection tyrosine phosphorylation. 4G10 is well known for its sensitivity and its ability to detect multiple tyrosine phosphorylations on numerous substrates. It has been validated by thousands of scientific and medical researchers in virtually every application and tyrosine target over the past 2 decades. Now Millipore has made the best even better with 4G10 Platinum. We pooled 4G10 with the next most highly regarded anti-phosphotyrosine, clone PY20, to make 4G10 Platinum. PY20 itself is a very poor substitute for 4G10, but its additive effect allow for a greater level of detection on more substrates that even 4G10 alone is capable.
Immunogen
The immunogen for 4G10 was phospho-tyramine coupled to KLH, while the immunogen for PY20 was phosphotyrosine conjugated to a carrier protein.
Anwendung
4G10 Platinum, Anti-Phosphotyrosine, Agarose Conjugate detects levels of Phosphotyrosine proteins & has been published & validated for use in IP.
Leistungsmerkmale und Vorteile
Format: Gel Immobilized
Qualität
Immunoprecipitation:
Twenty μl of 50% slurry of this reagent (10 μl packed beads) was used to immunoprecipitate tyrosine-phosphorylated proteins from 250 μg PDGF-stimulated NIH3T3 cell lysate or EGF-stimulated A431 cell lysate.
Twenty μl of 50% slurry of this reagent (10 μl packed beads) was used to immunoprecipitate tyrosine-phosphorylated proteins from 250 μg PDGF-stimulated NIH3T3 cell lysate or EGF-stimulated A431 cell lysate.
Zielbeschreibung
Dependent upon the molecular weight of the Tyrosine-phosphorylated protein
Physikalische Form
protein G purified mouse monoclonal 4G10 (IgG2bκ) and protein A purified PY20 (IgG2b)
Supplied as a 50% slurry of a proprietary mixture of agarose conjugated, protein G purified mouse monoclonal 4G10 (IgG2bκ) and protein A purified PY20 (IgG2b) in PBS, pH 7.4 with 0.05% sodium azide.
Lagerung und Haltbarkeit
Stable for 6 months at 2-8°C from date of shipment. For maximum recovery of product, centrifuge the vial prior to removing the cap.
NOTE: DO NOT FREEZE.
NOTE: DO NOT FREEZE.
Rechtliche Hinweise
4G10 is a registered trademark of Upstate Group, Inc.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Lagerklassenschlüssel
10 - Combustible liquids
WGK
WGK 2
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S Ruff-Jamison et al.
The Journal of biological chemistry, 266(10), 6607-6613 (1991-04-15)
Phosphotyrosine and similar analogs have been used to elicit antibodies that have found widespread use in the study of cellular tyrosine phosphorylation. In order to better understand the anti-phosphotyrosine immune response and to elucidate the details of the specific association
Francesco E Boccalatte et al.
Blood, 113(12), 2776-2790 (2008-10-11)
Anaplastic large cell lymphoma represents a subset of neoplasms caused by translocations that juxtapose the anaplastic lymphoma kinase (ALK) to dimerization partners. The constitutive activation of ALK fusion proteins leads to cellular transformation through a complex signaling network. To elucidate
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Cancer research, 67(9), 4278-4286 (2007-05-08)
Anaplastic large cell lymphomas (ALCL) are mainly characterized by the reciprocal translocation t(2;5)(p23;q35) that involves the anaplastic lymphoma kinase (ALK) gene and generates the fusion protein NPM-ALK with intrinsic tyrosine kinase activity. NPM-ALK triggers several signaling cascades, leading to increased
Rachit Badolia et al.
The Journal of biological chemistry, 292(35), 14516-14531 (2017-07-15)
Platelets play a key role in the physiological hemostasis or pathological process of thrombosis. Rhodocytin, an agonist of the C-type lectin-like receptor-2 (CLEC-2), elicits powerful platelet activation signals in conjunction with Src family kinases (SFKs), spleen tyrosine kinase (Syk), and
Philippe Foubert et al.
The Journal of clinical investigation, 117(6), 1527-1537 (2007-05-19)
Endothelial progenitor cell (EPC) transplantation has beneficial effects for therapeutic neovascularization; however, only a small proportion of injected cells home to the lesion and incorporate into the neocapillaries. Consequently, this type of cell therapy requires substantial improvement to be of
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