16-305

Anti-phospho-Akt1/PKBα (Ser473) Antibody, clone 11E6, Alexa Fluor^™ 488

clone 11E6, Upstate^®, from mouse

• Synonym(e): PKB, PKD-alpha, AKT1, Protein Kinase B
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: ALEXA FLUOR^™ 488
• Clone: 11E6, monoclonal
• Application: FACS, ICC, IF, WB
• Citations: -

Eigenschaften

• biological source: mouse
• Quality Segment: 100
• conjugate: ALEXA FLUOR^™ 488
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 11E6, monoclonal
• species reactivity: rat, canine, human, mouse
• manufacturer/tradename: Upstate^®
• technique(s): flow cytometry: suitable, immunocytochemistry: suitable, immunofluorescence: suitable, western blot: suitable
• isotype: IgG1
• NCBI accession no.: NM_001014431.1, NM_001014432.1, NM_005163.2
• UniProt accession no.: P31749
• shipped in: wet ice
• target post-translational modification: phosphorylation (pSer473)

Beschreibung

General description

60 kDa

Immunogen

KLH conjugated synthetic peptide containing a pSer that corresponds to amino acid residues around phosphoserine 473.

Application

Detect phospho-Akt1/PKBα (Ser473) using this Anti-Akt1 Antibody, clone 11E6, Alexa Fluor 488 validated for use in FC, IC, IF & WB.

Research Category
Signaling

Research Sub Category
PI3K, Akt, & mTOR Signaling

Biochem/physiol Actions

Recognizes phosphorylatedAkt1/PKBα at Ser473. This lot of antibody does not cross-react with Akt1/PKBα dephosphorylated at Ser473.

Physical form

Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography

Preparation Note

1 year at 4°C from date of shipment

Analysis Note

Routinely evaluated by immunocytochemistry and flow cytometry.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Alexa Fluor is a registered trademark of Molecular Probes, Inc.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable