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Merck

1.50451

Supelco

SeQuant® ZIC®-HILIC 200Å (5 µm) HPLC Columns

L × I.D. 50 mm x 4.6 mm HPLC Column, PEEK-lined stainless steel

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About This Item

UNSPSC-Code:
41115709

product name

SeQuant® ZIC-HILIC (5µm,200Å) PEEK 50 x 4.6 mm, suitable for HPLC

Materialien

PEEK-lined stainless steel column

Qualitätsniveau

Agentur

suitable for USP L114

Produktlinie

SeQuant®

Leistungsmerkmale

endcapped: no

Parameter

500 bar pressure
70 °C max. temp.

Methode(n)

HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)

Säule L × ID

5 cm × 4.6 mm

Matrix

fully porous particle

Aktive Matrixgruppe

ZIC-HILIC bonding phase

Partikelgröße

5 μm

Porengröße

200 Å pore size

Betriebs-pH-Wert

2-7.5

Trenntechnik

hydrophilic interaction (HILIC)

Lagertemp.

no temp limit

Allgemeine Beschreibung

SeQuant® ZIC®-HILIC Columns are designed to retain and separate all types of polar and hydrophilic compounds and for robust chromatography with high selectivity and reproducibility.
The silica-based ZIC®-HILIC sorbent has a bonded stationary phase consisting of a highly polar, permanent zwitterion. Separation selectivity is favored by the 1:1 zwitterion charge balance, which makes the ZIC®-HILIC Column overall neutral, with weak, but important, ionic interactions. Tuning of the selectivity on the ZIC®-HILIC Column during method development is facilitated by the pH-inpendent permanent zwitterion, ensuring that only the analytes (and not the column) are affected during eluent optimization.
The Pore size of 200 Å makes this column perfectly suitable for the separation of larger polar molecules under HILIC conditions.
The column dimension of 50 mm length x 4.6 mm ID is the best choice for the fast separation of polar columns using a standard HPLC Instrument.
Thanks to their favorable retention and robust performance, SeQuant® HILIC columns have become extremely popular in numerous laboratories around the world. For example, US FDA (United States Food and Drug Administration) has recommended SeQuant® ZIC®-HILIC for analysis of melamine and related substances. SeQuant® HILIC columns excel in stability and low bleed. These features make them particularly suitable for LC-MS applications where high background can lead to signal suppression and interference with quantitative measurements while also increasing instrument wear. Their high stability makes these high-performance HILIC columns also attractive for traditional HPLC applications.
Learn more about the Supelco HPLC Column portfolio and Method development:
Brochure: HPLC and UHPLC Column Selection Guide
Brochure: A Practical Guide to HPLC - Method Development Guide

Hinweis zur Analyse

Theoretische Bodenzahl (N/m) (Cytosine): ≥ 60000
Symmetrie (Cytosine): 0.95 - 1.40

Rechtliche Hinweise

SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC-HILIC is a trademark of Merck KGaA, Darmstadt, Germany

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