1.50451
SeQuant® ZIC®-HILIC 200Å (5 µm) HPLC Columns
L × I.D. 50 mm x 4.6 mm HPLC Column, PEEK-lined stainless steel
About This Item
Empfohlene Produkte
product name
SeQuant® ZIC-HILIC™ (5µm,200Å) PEEK 50 x 4.6 mm, suitable for HPLC
Materialien
PEEK-lined stainless steel column
Qualitätsniveau
Agentur
suitable for USP L114
Produktlinie
SeQuant®
Leistungsmerkmale
endcapped: no
Parameter
500 bar pressure
70 °C max. temp.
Methode(n)
HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)
Säule L × ID
5 cm × 4.6 mm
Matrix
fully porous particle
Aktive Matrixgruppe
ZIC-HILIC bonding phase
Partikelgröße
5 μm
Porengröße
200 Å pore size
Betriebs-pH-Wert
2-7.5
Trenntechnik
hydrophilic interaction (HILIC)
Lagertemp.
no temp limit
Allgemeine Beschreibung
The silica-based ZIC®-HILIC sorbent has a bonded stationary phase consisting of a highly polar, permanent zwitterion. Separation selectivity is favored by the 1:1 zwitterion charge balance, which makes the ZIC®-HILIC Column overall neutral, with weak, but important, ionic interactions. Tuning of the selectivity on the ZIC®-HILIC Column during method development is facilitated by the pH-inpendent permanent zwitterion, ensuring that only the analytes (and not the column) are affected during eluent optimization.
The Pore size of 200 Å makes this column perfectly suitable for the separation of larger polar molecules under HILIC conditions.
The column dimension of 50 mm length x 4.6 mm ID is the best choice for the fast separation of polar columns using a standard HPLC Instrument.
Thanks to their favorable retention and robust performance, SeQuant® HILIC columns have become extremely popular in numerous laboratories around the world. For example, US FDA (United States Food and Drug Administration) has recommended SeQuant® ZIC®-HILIC for analysis of melamine and related substances. SeQuant® HILIC columns excel in stability and low bleed. These features make them particularly suitable for LC-MS applications where high background can lead to signal suppression and interference with quantitative measurements while also increasing instrument wear. Their high stability makes these high-performance HILIC columns also attractive for traditional HPLC applications.
Learn more about the Supelco HPLC Column portfolio and Method development:
Brochure: HPLC and UHPLC Column Selection Guide
Brochure: A Practical Guide to HPLC - Method Development Guide
Hinweis zur Analyse
Symmetrie (Cytosine): 0.95 - 1.40
Rechtliche Hinweise
Analysenzertifikate (COA)
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Protokolle
Separation of EDTA
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