14-935-M

GRK1, active

• UNSPSC Code: 12352204
• NACRES: NA.32
• eCl@ss: 32160405
• Biological source: human

Eigenschaften

• biological source: human
• Quality Segment: 100
• mol wt: M_w 68 kDa
• manufacturer/tradename: Upstate^®
• technique(s): activity assay: suitable (kinase)
• NCBI accession no.: NP_002920
• UniProt accession no.: Q15835

Beschreibung

General description

Product Source: N-terminal 6His-tagged, recombinant, human GRK1 full length, expressed by baculovirus in Sf21 insect cells

Retina-specific kinase involved in the signal turnoff via phosphorylation of rhodopsin (RHO), the G protein- coupled receptor that initiates the phototransduction cascade. This rapid desensitization is essential for scotopic vision and permits rapid adaptation to changes in illumination.

Biochem/physiol Actions

Target Sub-Family: AGC

Packaging

Also available in 250μg size as catalog # 14-935M and in bulk as catalog # 14-935-K.

Physical form

Protein in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.

Purified using immobilized metal affinity chromatography.

Preparation Note

6 months at -70°C

Analysis Note

Routinely evaluated by phosphorylation of KKKKERLLDDRHDSGLDSMKDEE

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• pictograms: GHS07
• signalword: Warning
• hcodes: H317
• pcodes: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable