14-842-M

Lck Protein, activated, 10 µg

activated, N-terminal 6His-tagged, recombinant, human Lck full length, for use in Kinase Assays.

• UNSPSC-Code: 12352202
• eCl@ss: 32160405
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: human
• Qualitätsniveau: 100
• Rekombinant: expressed in baculovirus infected Sf21 cells
• Produktlinie: Upstate^®
• Spezifische Aktivität: (For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.)
• Mol-Gew.: M_w 59 kDa
• Aufgereinigt durch: (Ni²⁺/NTA-agarose)
• Speziesreaktivität: human
• Hersteller/Markenname: Upstate^®
• Methode(n): activity assay: suitable (kinase)
• Hinterlegungsnummer: (FUNCTION: SwissProt: P06239 # Tyrosine kinase that plays an essential role for the selection and maturation of developing T-cell in the thymus and in mature T-cell function. Is constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors and plays a key role in T-cell antigen receptor(TCR)-linked signal transduction pathways. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, and thereby recruits the associated LCK to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosines-based activation motifs (ITAMs) in the cytoplasmic tails of the TCRgamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. In addition, contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, and upon engagement of the CD2 molecule, LCK undergoes hyp); NM_005356.3
• NCBI-Hinterlegungsnummer: NM_005356.3
• UniProt-Hinterlegungsnummer: P06239
• Relevante Krankheit(en): obesity; metabolic disorders

Beschreibung

Allgemeine Beschreibung

N-terminal 6His-tagged, recombinant, human Lck full length

Anwendung

Research Category
Stoffwechsel

Auto-activated on column by incubating with Mg/ATP, excess ATP and MgAc removed by multiple column wash steps.

Research Sub Category
Obesity

Metabolic Disorders

Biochem./physiol. Wirkung

Protein Target: Lck

Target Sub-Family: TK

Verpackung

Also available in 10 μg size and in bulk

Also available in 250 µg size or bulk --call for pricing and availability and reference catalog number 14-842M when ordering the 250 µg size.

Zielbeschreibung

Lck

Verlinkung

Replaces: 14-710

Physikalische Form

Ni²⁺/NTA-agarose

Lagerung und Haltbarkeit

1 year at -70°C from date of shipment

Sonstige Hinweise

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Rechtliche Hinweise

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Piktogramme: GHS07
• Signalwort: Warning
• H-Sätze: H317
• P-Sätze: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Gefahreneinstufungen: Skin Sens. 1
• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°C): Not applicable