07-706

Anti-WDR5 Antibody

Upstate^®, from rabbit

• Synonym(e): BIG3, BMP2-induced 3-kb gene protein, Cilia and flagella associated protein 89, Set1c WD40 repeat protein homolog, SWD3, WD repeat-containing protein 5, WD-repeat protein 5
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: affinity purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: human
• Hersteller/Markenname: Upstate^®
• Methode(n): western blot: suitable
• Isotyp: IgG
• NCBI-Hinterlegungsnummer: NP_060058
• UniProt-Hinterlegungsnummer: P61964
• Versandbedingung: dry ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... WDR5(11091)

Beschreibung

Allgemeine Beschreibung

WD repeat-containing protein 5 (also known as BMP2-induced 3-kb gene protein, WD repeat-containing protein 5, WD-repeat protein 5, Set1c WD40 repeat protein homolog, cilia and flagella associated protein 89) is a nuclear protein encoded by the WDR5 (also known as BIG3, CFAP89, SWD3) gene in human. This protein contains 7 WD (Trp-Asp) repeats and belongs to the WD repeat protein family whose members are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. WD repeat-containing protein 5 is a component of several complexes that regulate histone methylation and acetylation levels, including ATAC (ADA2A-containing complex) complex, SET1 complex, MLL1/MLL, MLL2/3 (also named ASCOM complex), MLL4/WBP7, and NSL (non-specific lethal) complex.

Spezifität

Evaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: A 1:4,000 dilution of this antibody detected WDR5 in 10 µg of HeLa nuclear extract.

Recognizes WDR5, MW 36 kDa. A related protein band of 43 kDa that was blocked by the immunizing peptide was also detected. Identification of WDR5 protein at 36 kDa was confirmed with siRNA knockdown by an independent laboratory.

Immunogen

Epitope: Near N-terminus

KLH-conjugated linear peptide corresponding to the N-terminus of human WDR5.

Anwendung

Research Category
Epigenetik & nukleäre Funktionen

Research Sub Category
Histone

Detect WDR5 using this Anti-WDR5 Antibody validated for use in WB.

Western Blot: 0.05-0.25 μg/mL using HeLa nuclear extracts.

Qualität

Evaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: A 1:4,000 dilution of this antibody detected WDR5 in 10 µg of HeLa nuclear extract.

Zielbeschreibung

~39 kDa observed

Physikalische Form

Affinity purified

Purified rabbit polyclonal IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide with 30% glycerol.

Lagerung und Haltbarkeit

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Rechtliche Hinweise

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable