07-692
Anti-Sar1 Antibody
Upstate®, from rabbit
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eCl@ss:
32160702
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
WB
Citations:
11
Technischer Dienst
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Unterstützung erhaltenbiological source
rabbit
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
monkey, mouse, rat, human
manufacturer/tradename
Upstate®
technique(s)
western blot: suitable
isotype
IgG
NCBI accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ROR1(4919)
General description
Mr 21kDa
Immunogen
KLH-conjugated, synthetic peptide corresponding to amino acids 46-62 (CKDDRLGQHVPTLHPTSE) of human Sar1 (a and b isoforms), with an N-terminal cysteine added for conjugation purposes
Application
Detect Sar1 using this Anti-Sar1 Antibody validated for use in WB.
Research Category
Signaling
Signaling
Research Sub Category
G-proteins
Kinases & Phosphatases
G-proteins
Kinases & Phosphatases
Biochem/physiol Actions
Sar1
Physical form
Format: Purified
Protein A purified
Preparation Note
stable 2 years at -20°C from date of shipment
Analysis Note
Control
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
routinely evaluated by immunoblot on RIPA lysates from 3T3/A31 cells
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Lagerklasse
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Analysenzertifikate (COA)
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Bethan Jones et al.
Nature genetics, 34(1), 29-31 (2003-04-15)
Dietary fat is an important source of nutrition. Here we identify eight mutations in SARA2 that are associated with three severe disorders of fat malabsorption. The Sar1 family of proteins initiates the intracellular transport of proteins in COPII (coat protein)-coated
François-Xavier Campbell-Valois et al.
Molecular & cellular proteomics : MCP, 11(7), M111-M111 (2012-03-20)
Phagosomes, by killing and degrading pathogens for antigen presentation, are organelles implicated in key aspects of innate and adaptive immunity. Although it has been well established that phagosomes consist of membranes from the plasma membrane, endosomes, and lysosomes, the notion
A structural view of the COPII vesicle coat.
Bickford, Lincoln C, et al.
Current Opinion in Structural Biology, 14, 147-153 (2004)