07-645
Anti-Sp1-Antikörper
Upstate®, from rabbit
Synonym(e):
Sp1 transcription factor, specificity protein 1
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Über diesen Artikel
biological source
rabbit
Quality Segment
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, human, rat
packaging
antibody small pack of 25 μg
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable, electrophoretic mobility shift assay: suitable, immunofluorescence: suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... SP1(6667)
General description
Immunogen
Application
Transkriptionsfaktoren
Epigenetik & Zellkernfunktion
4 µg dieser Charge immunpräzipitierten Sp1 aus 500 µg HeLa-Zellkernextrakt.
HINWEIS: Eine frühere Charge immunpräzipitierte die α-1-Untereinheit mit dem Catch and Release v2.0 Reversible Immunoprecipitation System (Katalognr. 17-500) erfolgreich. Weitere Informationen und das Protokoll finden Sie im Handbuch: www.millipore.com
Chromatin-Immunpräzipitation:
Von einem unabhängigen Labor berichtet.
Immunfluoreszenz:
Von einem unabhängigen Labor berichtet.
Electrophoretic Mobility Shift Assay (EMSA):
Von einem unabhängigen Labor wurde berichtet, dass 1 µl einer früheren Charge dieses Antikörpers an DNA-Komplexen, die Sp1 und endogenes Sp1 aus HeLa-Zellen enthalten, in EMSA einen Supershift durchführen konnten.
Biochem/physiol Actions
Physical form
Preparation Note
Analysis Note
Positive Antigenkontrolle: Best.-Nr. 12-309, HeLa-Zellkern-Extrakt. Geben Sie das gleiche Volumen reduzierenden Laemmli-Probenpuffers zu 10 μl Extrakt und kochen Sie das Gemisch 5 Minuten lang ein. Beladen Sie 20 μg reduziertes Extrakt je Bahn für Minigele.
Western-Blot-Analyse:
Eine 1:500-Verdünnung dieser Charge wies Sp1 in 10 μg HeLa-Lysat nach.
Other Notes
Legal Information
Disclaimer
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Lagerklasse
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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Verwandter Inhalt
Cancer is a complex disease manifestation. At its core, it remains a disease of abnormal cellular proliferation and inappropriate gene expression. In the early days, carcinogenesis was viewed simply as resulting from a collection of genetic mutations that altered the gene expression of key oncogenic genes or tumor suppressor genes leading to uncontrolled growth and disease (Virani, S et al 2012). Today, however, research is showing that carcinogenesis results from the successive accumulation of heritable genetic and epigenetic changes. Moreover, the success in how we predict, treat and overcome cancer will likely involve not only understanding the consequences of direct genetic changes that can cause cancer, but also how the epigenetic and environmental changes cause cancer (Johnson C et al 2015; Waldmann T et al 2013). Epigenetics is the study of heritable gene expression as it relates to changes in DNA structure that are not tied to changes in DNA sequence but, instead, are tied to how the nucleic acid material is read or processed via the myriad of protein-protein, protein-nucleic acid, and nucleic acid-nucleic acid interactions that ultimately manifest themselves into a specific expression phenotype (Ngai SC et al 2012, Johnson C et al 2015). This review will discuss some of the principal aspects of epigenetic research and how they relate to our current understanding of carcinogenesis. Because epigenetics affects phenotype and changes in epigenetics are thought to be key to environmental adaptability and thus may in fact be reversed or manipulated, understanding the integration of experimental and epidemiologic science surrounding cancer and its many manifestations should lead to more effective cancer prognostics as well as treatments (Virani S et al 2012).
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 07-645-25UG | 04054839337390 |