06-702
Anti-phospho-Smad1 (Ser463/465) Antibody
Upstate®, from rabbit
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Über diesen Artikel
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
polyclonal
Species reactivity:
human, Xenopus, rat, mouse
Application:
WB
Citations:
29
General description
65 kDa
Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad), function downstream of TGF-β receptor Serine/Threonine kinases and undergo serine phosphorylation in response to receptor activation. Following BMP (bone morphogenic protein) or TGF-β binding to the targeted surface receptors, Smad1 (also designated Madr1 or JV4-1) becomes phosphorylated at Ser 463 and Ser 465. Activated Smad1 associates with Smad4 and translocates to the nucleus, where the Smad1/Smad4 complex recruits DNA-binding proteins to activate specific gene transcription. BMP binding induces phosphorylation of Smad1, which enhances the binding of Smad1 to CBP to stimulate Smad1-dependent transcription. In addition, ALK1 and ALK2 specifically phosphorylate Smad1 on residues located in the MH2 domain and induces Smad1-dependent pathways.
Immunogen
peptide corresponding to (CGGSPHNPISpSVpSGG) corresponding to amino acids 455-465 of human Smad1 protein
Application
Detect phospho-Smad1 (Ser463/465) with Anti-phospho-Smad1 (Ser463/465) Antibody (Rabbit Polyclonal Antibody), that has been shown to work in WB.
Biochem/physiol Actions
Recognizes the dual serine phosphorylated Smad1 (Ser463/465).
Physical form
Format: Purified
Analysis Note
Control
BMP-4-treated HeLa or NIH/3T3 cells
BMP-4-treated HeLa or NIH/3T3 cells
routinely evaluated by immunoblot on RIPA lysates from P19 (mouse embryonal carcinoma) cells treated with BMP4
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
TGFbeta-mediated activation of Smad1 in B-cell non-Hodgkin's lymphoma and effect on cell proliferation.
Munoz, O; Fend, F; de Beaumont, R; Husson, H; Astier, A; Freedman, AS
Leukemia null
Peiwen Wu et al.
American journal of physiology. Renal physiology, 312(6), F1090-F1100 (2017-03-17)
Collagen IV (Col IV) is a major component of expanded glomerular extracellular matrix in diabetic nephropathy and Smad1 is a key molecule regulating Col IV expression in mesangial cells (MCs). The present study was conducted to determine if Smad1 pathway
BMP signaling dynamics in embryonic orofacial tissue.
Partha Mukhopadhyay,Cynthia L Webb,Dennis R Warner,Robert M Greene,M Michele Pisano
Journal of Cellular Physiology null
Interaction between Smad1 and p97/VCP in rat testis and epididymis during the postnatal development.
Sevil Cayli,Fikret Erdemir,Seda Ocakli,Bahadir Ungor,Hakan Kesici,Tamer Yener,Huseyin Aslan
Reproductive Sciences null
Signal transduction. Mad about SMADs.
J Wrana et al.
Nature, 388(6637), 28-29 (1997-07-03)
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