This product is considered a PCR reagent, as are a number of other products and compounds. The term ‘Reagent’, in this context, simply mean a component or substance in a given chemical reaction or method. For PCR, reagents would include this PCR tested water, as well as Taq polymerase, primers, dNTPs, buffers, Magnesium Chloride, and template DNA. Please see the link below to review available PCR Kits and Reagents:
https://www.sigmaaldrich.com/products/molecular-biology-and-functional-genomics/pcr-and-amplification/pcr-reagents-and-kits
W1754
Water
PCR Reagent, suitable for PCR
Synonym(s):
H2O
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About This Item
Linear Formula:
H2O
CAS Number:
Molecular Weight:
18.02
NACRES:
NA.25
PubChem Substance ID:
UNSPSC Code:
12191602
EC Number:
231-791-2
MDL number:
Beilstein/REAXYS Number:
2050024
Technique(s):
PCR: suitable
Bp:
100 °C (lit.)
Vapor pressure:
3 mmHg
Pricing and availability is not currently available.
Product Name
Water, PCR Reagent
vapor density
<1 (vs air)
Quality Segment
vapor pressure
3 mmHg
sterility
sterile-filtered
form
liquid
packaging
vial of 1.5 mL
technique(s)
PCR: suitable
refractive index
n20/D 1.34 (lit.)
pH
5-7
bp
100 °C (lit.)
mp
0 °C (lit.)
density
1.000 g/mL at 3.98 °C (lit.)
foreign activity
DNase, none detected, RNase, none detected
SMILES string
O
InChI
1S/H2O/h1H2
InChI key
XLYOFNOQVPJJNP-UHFFFAOYSA-N
General description
PCR grade water is sterile-filtered. It is free from exonucleases (DNAse, RNAse) and endonuclease (NICKase) and is also free from nucleic acid contamination.
Application
Suitable for polymerase chain reaction (PCR)
Water has been used:
- as a component of the reaction mixture and as a diluting agent in microfluidic RT-qPCR
- as a component of the reaction mixture for the amplification of products from fungal (Trametes versicolor) DNA
- as a diluting agent and as a component of the reaction mixture for the amplification of cDNA[1]
- Water has been used to make up the final volume of the sample in polymerase chain reaction (PCR)[1]
Other Notes
Easily compare specifications for Water products with the Water specification table.
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This Item | |||
|---|---|---|---|
| Quality Level 200 | Quality Level 100 | Quality Level 100 | Quality Level 300 |
| technique(s) PCR: suitable | technique(s) FISH: suitable | technique(s) HPLC: suitable | technique(s) - |
| form liquid | form liquid | form liquid | form liquid |
| pH 5-7 | pH 5-7 | pH - | pH - |
| vapor pressure 3 mmHg | vapor pressure - | vapor pressure 23 hPa ( 20 °C) | vapor pressure - |
| density 1.000 g/mL at 3.98 °C (lit.) | density 1.000 g/mL at 3.98 °C (lit.) | density 1.000 g/mL at 3.98 °C (lit.) | density 1.000 g/mL at 3.98 °C (lit.) |
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Storage Class
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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Is water the pcr reagent? Or both are different?
1 answer-
Helpful?
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容量がわかりません。型番の1 VLとは1000 mLのことですか？
1 answer-
As mentioned in the 'PROPERTIES' section, the volume of each vial is 1.5 mL.
Helpful?
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