S7388

N-Succinyl-Ala-Ala-Pro-Phe p-nitroanilide

Subtilisin substrate

• Synonym(s): N-Succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine 4-nitroanilide, N-Succinyl-Ala-Ala-Pro-Phe-pNA
• Empirical Formula (Hill Notation): C₃₀H₃₆N₆O₉
• CAS Number: 70967-97-4
• Molecular Weight: 624.64
• NACRES: NA.32
• PubChem Substance ID: 24899742
• UNSPSC Code: 12352200
• MDL number: MFCD00077161
• Beilstein/REAXYS Number: 4289896

Properties

• Product Name: N-Succinyl-Ala-Ala-Pro-Phe p-nitroanilide,
• Quality Segment: 300
• storage temp.: −20°C
• SMILES string: [H][C@]1(CCCN1C(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)CCC(O)=O)C(=O)N[C@@H](Cc2ccccc2)C(=O)Nc3ccc(cc3)[N+]([O-])=O
• InChI: 1S/C30H36N6O9/c1-18(31-25(37)14-15-26(38)39)27(40)32-19(2)30(43)35-16-6-9-24(35)29(42)34-23(17-20-7-4-3-5-8-20)28(41)33-21-10-12-22(13-11-21)36(44)45/h3-5,7-8,10-13,18-19,23-24H,6,9,14-17H2,1-2H3,(H,31,37)(H,32,40)(H,33,41)(H,34,42)(H,38,39)/t18-,19-,23-,24-/m0/s1
• InChI key: LKDMKWNDBAVNQZ-WJNSRDFLSA-N
• Gene Information: human ... FKBP10(60681)

Description

General description

N-Succinyl-Ala-Ala-Pro-Phe p-nitroanilide is a colorimetric substrate for chymotrypsin, human leukocyte cathepsin G and peptidyl prolyl isomerase.

Application

N-Succinyl-Ala-Ala-Pro-Phe p-nitroanilide has been used as a substrate to determine chymotrypsin activity.

N-Succinyl-Ala-Ala-Pro-Phe p-nitroanilide has been used:

• as a substrate for cathepsin G assay in bone marrow lysates.
• in proteolytic activity assay of serine protease subtilisin carlsberg (SC).
• as a substrate for neutrophil elastase, trypsin and chymotrypsin in protease assay.

Biochem/physiol Actions

N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide is a substrate for subtilisin and proteases.[1]

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)