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Phospholipase A₂ from bovine pancreas

Name: Phospholipase A<SUB>2</SUB> from bovine pancreas
Brand: SIGMA

lyophilized powder, ≥20 units/mg protein

Synonym(s):

Lecithinase A, PLA₂, Phosphatidylcholine 2-acylhydrolase

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About This Item

CAS Number:

9001-84-7

UNSPSC Code:

12352204

NACRES:

NA.32

EC Number:

232-637-7

MDL number:

MFCD00131890

EC Number:

3.1.1.4 (BRENDA, IUBMB)

Specific activity:

≥20 units/mg protein

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Properties

form

lyophilized powder

Quality Segment

200

specific activity

≥20 units/mg protein

composition

Protein, 5-20% biuret

UniProt accession no.

P00593

storage temp.

−20°C

Gene Information

cow ... PLA2G1B(282457)

Description

General description

Phospholipase A2 from bovine pancreas is a calcium-dependent lipolytic enzyme.

Application

Phospholipase A2 from bovine pancreas has been used to determine rat renal proximal tubular segments (PTS) viability during oxygenation and hypoxia-reoxygenation and to study the impact of phospholipase A2 on tubular cell triglyceride levels.

Biochem/physiol Actions

Hydrolyzes the β-ester bond of zwitterionic glycerophospholipids. Preferred substrates are phosphatidylcholine, phosphatidylethanolamine, and their plasmalogen analogues. Phosphatidylinositol and phosphatidylserine are also hydrolyzed. Aggressively attacks phospholipids in membranes of intact cells.

Packaging

Package size based on protein content

Physical form

Contains Tris buffer salts.

Analysis Note

Protein determined by biuret.

Other Notes

One unit will hydrolyze 1.0 μmole of soybean L-α-phosphatidylcholine to L-α-lysophosphatidylcholine and a fatty acid per min at pH 8.0 and 37 °C.

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This Item	T6763	C4129	C7762
Sigma-Aldrich P8913 Phospholipase A₂ from bovine pancreas	Sigma-Aldrich T6763 Trypsin Acetylated from bovine pancreas	Sigma-Aldrich C4129 α-Chymotrypsin from bovine pancreas	Sigma-Aldrich C7762 α-Chymotrypsin from bovine pancreas
description lyophilized powder, ≥20 units/mg protein	description Type V-S, ≥8,500 BAEE units/mg protein (biuret)	description Type II, lyophilized powder, ≥40 units/mg protein	description Type I-S, essentially salt-free, lyophilized powder
Gene Information cow ... PLA2G1B(282457)	Gene Information cow ... TRYP8(282603)	Gene Information cow ... CTRB1(618826)	Gene Information cow ... CTRB1(618826)
specific activity ≥20 units/mg protein	specific activity ≥8,500 BAEE units/mg protein (biuret)	specific activity ≥40 units/mg protein	specific activity ≥40 units/mg protein
form lyophilized powder	form powder	form lyophilized powder	form essentially salt-free, lyophilized powder
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
UniProt accession no. P00593	UniProt accession no. Q29463	UniProt accession no. P00767	UniProt accession no. P00767
Quality Level 200	Quality Level 200	Quality Level 300	Quality Level 200

WGK

WGK 1

Storage Class

11 - Combustible Solids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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