P6534

Phospholipase A₂ from porcine pancreas

ammonium sulfate suspension, ≥600 units/mg protein

• Synonym(s): Lecithinase A, PLA₂, Phosphatidylcholine 2-acylhydrolase
• CAS Number: 9001-84-7
• UNSPSC Code: 12352204
• NACRES: NA.32
• EC Number: 232-637-7
• MDL number: MFCD00131890
• EC Number: 3.1.1.4 (BRENDA, IUBMB)
• Specific activity: ≥600 units/mg protein

Properties

• form: ammonium sulfate suspension
• Quality Segment: 200
• specific activity: ≥600 units/mg protein
• UniProt accession no.: P00592
• storage temp.: 2-8°C
• Gene Information: pig ... PLA2G1B(445525)

Description

General description

Phospholipase A2 is a small disulfide-rich protein having 124 residues. It is a calcium-dependent enzyme.

Application

Phospholipase A2 has been used in phospholipase assay and to determine rat renal proximal tubular segments (PTS) viability during oxygenation and hypoxia-reoxygenation.

Biochem/physiol Actions

It has a high catalytic activity on aggregated substrates compared to monomeric substrates.

Hydrolyzes the β-ester bond of zwitterionic glycerophospholipids. Preferred substrates are phosphatidylcholine, phosphatidylethanolamine, and their plasmalogen analogues. Phosphatidylinositol and phosphatidylserine are also hydrolyzed. Aggressively attacks phospholipids in membranes of intact cells.

Physical form

Suspension in 3.2 M (NH₄)₂SO₄ solution, pH 5.5

Analysis Note

Protein determined by biuret.

Other Notes

One unit will hydrolyze 1.0 μmole of soybean L-α-phosphatidylcholine to L-α-lysophosphatidylcholine and a fatty acid per min at pH 8.0 at 37 °C.

Safety Information

• Storage Class: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable