Skip to Content
MilliporeSigma
All Photos(1)

Documents

P1291

Sigma-Aldrich

Peroxidase Anti-Peroxidase Soluble Complex antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Antiperoxidase, Pap Antibody, Peroxidase Anti Peroxidase, Peroxidase Anti Peroxidase - Peroxidase Anti-Peroxidase Soluble Complex antibody produced in rabbit, PAP

Sign Into View Organizational & Contract Pricing


About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

form

buffered aqueous solution

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Looking for similar products? Visit Product Comparison Guide

Specificity

By immunoelectrophoresis (IEP), a single arc of precipitation is observed when the product (prior to the addition of BSA) is electrophoresed and then allowed to diffuse against anti-rabbit whole serum or anti-rabbit IgG. No reaction is observed against free horseradish peroxidase.

Application

Peroxidase Anti-Peroxidase Soluble Complex antibody produced in rabbit has been used in:
  • immunoblotting
  • to detect the expression of TAP-tagged proteins
  • immunoprecipitation
  • immunohistochemical staining

Peroxidase Anti-Peroxidase Soluble Complex antibody produced in rabbit was used in immunoblotting of yeast whole cell extracts.
Sigma PAP is assayed by immunoelectrophoresis, Ouchterlony double diffusion, and immunohistochemical staining techniques to ensure purity and functional reactivity. It is recommended that the optimal working dilution be determined in the specific assay system.
Sigma Peroxidase Anti-Peroxidase Soluble Complex antibody produced in rabbit is assayed by immunoelectrophoresis, Ouchterlony double diffusion, and immunohistochemical staining techniques to ensure purity and functional reactivity. It is recommended that the optimal working dilution be determined in the specific assay system.

Biochem/physiol Actions

Peroxidase:anti-peroxidase (PAP) immune complex is inexpensive immunoglobulin ligand. It is widely used for the detection of fragment crystallisable (Fc) receptor binding and fragment antigen binding (Fab) regions.
The Peroxidase Anti-Peroxidase (PAP) soluble complex is prepared by a modification of the method of Sternberger using specific polyclonal or monoclonal antibodies to horseradish peroxidase and highly purified enzyme. Since the enzyme is not covalently coupled to the antibody, the soluble complex retains its enzyme and antibody reactivity with distinct advantages over enzyme-conjugated antibodies.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT

Storage and Stability

Store at –20 °C. For continuous use, the product may be stored at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots at –20 °C. Repeated freezing and thawing or storage in "frost-free" freezers, is notrecommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Skin Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Vladimir Yong-Gonzales et al.
PloS one, 7(12), e51540-e51540 (2013-01-04)
The Smc5-Smc6 complex in Saccharomyces cerevisiae is both essential for growth and important for coping with genotoxic stress. While it facilitates damage tolerance throughout the genome under genotoxin treatment, its function during unperturbed growth is mainly documented for repetitive DNA
Distribution and relative frequency of immunohistochemically detected endocrine cells in the stomach of New Zealand White rabbit (Oryctolagus cuniculus)
Turk S, et al.
Iranian journal of veterinary research, 20(1), 39-45 (2019)
Amparo Cuéllar Pérez et al.
PloS one, 9(1), e84891-e84891 (2014-01-15)
Jasmonate (JA) signalling is mediated by the JASMONATE-ZIM DOMAIN (JAZ) repressor proteins, which are degraded upon JA perception to release downstream responses. The ZIM protein domain is characteristic of the larger TIFY protein family. It is currently unknown if the
Åsa Segerstolpe et al.
Nucleic acids research, 41(2), 1178-1190 (2012-11-30)
Ribosomal subunit biogenesis in eukaryotes is a complex multistep process. Mrd1 is an essential and conserved small (40S) ribosomal subunit synthesis factor that is required for early cleavages in the 35S pre-ribosomal RNA (rRNA). Yeast Mrd1 contains five RNA-binding domains
Roger Palou et al.
Cell cycle (Georgetown, Tex.), 14(24), 3842-3850 (2015-07-16)
The mitotic cell cycle is driven by Cyclin-Dependent Kinases (CDK). CDK activation requires the binding of activatory subunits termed cyclins. Different waves of cyclins are expressed during the cell cycle, enabling CDKs to trigger phase specific events. For instance, S

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service