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Minimum Essential Medium Eagle

Name: Minimum Essential Medium Eagle
Brand: SIGMA

With Earle′s salts and reduced NaHCO₃. without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

EMEM, MEM

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About This Item

UNSPSC Code:

12352207

NACRES:

NA.75

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Properties

Product Name

Minimum Essential Medium Eagle, Modified, with Earle′s salts and reduced NaHCO₃. without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Segment

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

phenol red: 0.011 g/L
NaHCO₃: 0.85 g/L
glucose: 1.0 g/L
L-glutamine: no

shipped in

ambient

storage temp.

2-8°C

Description

General description

Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed they had specific nutritional requirements that could not be met by Eagle′s Basal Medium (BME). Subsequent studies using these and other cells in culture indicated additions to BME could be made to aid growth of a wider variety of fastidious cells.

MEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has broadened the usefulness of this medium.

Application

Recommended for use in a carbon dioxide-free culture system.

Preparation Note

Supplement with 0.292 g/L L-glutamine.

Other Notes

May also be supplemented with HEPES to raise the buffering range.

This product lacks L-Ala; L-Asn; L-Glu; Gly; L-Pro; L-Ser and L-Gln.

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This Item	M2279	M5650	M0200
Sigma-Aldrich M2414 Minimum Essential Medium Eagle	Sigma-Aldrich M2279 Minimum Essential Medium Eagle	Sigma-Aldrich M5650 Minimum Essential Medium Eagle	Sigma-Aldrich M0200 Minimum Essential Medium Eagle
description Modified, with Earle′s salts and reduced NaHCO₃. without L-glutamine, liquid, sterile-filtered, suitable for cell culture	description With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture	description With Earle′s salts, non-essential amino acids and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture	description Alpha Modification, With sodium bicarbonate and L-glutamine, without ribonucleosides and deoxyribonucleosides, liquid, sterile-filtered, suitable for cell culture
sterility sterile-filtered	sterility sterile-filtered	sterility sterile-filtered	sterility sterile-filtered
form liquid	form liquid	form liquid	form liquid
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
components phenol red: 0.011 g/L NaHCO₃: 0.85 g/L glucose: 1.0 g/L L-glutamine: no	components phenol red: yes sodium pyruvate: no L-glutamine: no HEPES: no NaHCO₃: yes Earle’s salts (5% CO₂): yes	components phenol red: yes NaHCO₃: yes L-glutamine: no sodium pyruvate: no HEPES: no Earle’s salts (5% CO₂): yes	components phenol red: 0.011 g/L glucose: 1.0 g/L L-glutamine: 0.292 g/L NaHCO₃: 2.2 g/L
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C

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Storage Class

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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