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Minimum Essential Medium Eagle

Name: Minimum Essential Medium Eagle
Brand: SIGMA

With non-essential amino acids, Earle′s salts and sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

EMEM, MEM

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About This Item

UNSPSC Code:

12352207

NACRES:

NA.75

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Properties

Product Name

Minimum Essential Medium Eagle, With Earle′s salts, non-essential amino acids and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Segment

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

phenol red: yes
NaHCO₃: yes
L-glutamine: no
sodium pyruvate: no
HEPES: no
Earle’s salts (5% CO₂): yes

shipped in

ambient

storage temp.

2-8°C

Description

General description

Minimum Essential Medium Eagle (MEM) is a synthetic cell culture media, developed by Harry Eagle. It has higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has enhanced the uses of this medium. This formulation has been further modified by the optional elimination of calcium to permit the growth of cells in suspension culture. MEM can be used for the cultivation of a wide variety of cells grown in monolayers.

Application

Minimum Essential Medium Eagle has been used to culture:

HeLa cells[1]
Madin-Darby canine kidney (MDCK) cells
chondrosarcoma cell line[1]

This medium may be preferred for the growth of adherent cells in the presence of reduced serum or as a base for the development of a serum-free medium.

Preparation Note

Supplement with 0.292 g/L L-glutamine.

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This Item	M2279	M0644	M4526
Sigma-Aldrich M5650 Minimum Essential Medium Eagle	Sigma-Aldrich M2279 Minimum Essential Medium Eagle	Sigma-Aldrich M0644 Minimum Essential Medium Eagle	Sigma-Aldrich M4526 Minimum Essential Medium Eagle
sterility sterile-filtered	sterility sterile-filtered	sterility -	sterility sterile-filtered
form liquid	form liquid	form powder	form liquid
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
components phenol red: yes NaHCO₃: yes L-glutamine: no sodium pyruvate: no HEPES: no Earle’s salts (5% CO₂): yes	components phenol red: yes sodium pyruvate: no L-glutamine: no HEPES: no NaHCO₃: yes Earle’s salts (5% CO₂): yes	components NaHCO₃: no L-glutamine: yes phenol red: yes HEPES: no sodium pyruvate: yes	components Earle’s salts (5% CO₂): yes sodium pyruvate: yes glucose: yes L-glutamine: no HEPES: no Hanks’ salts (2% CO₂): no phenol red: yes NaHCO₃: yes stable glutamine: no
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C

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Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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