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Minimum Essential Medium Eagle

Name: Minimum Essential Medium Eagle
Brand: SIGMA

With Earle′s salts, non-essential amino acids, ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

EMEM, MEM

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About This Item

UNSPSC Code:

12352207

NACRES:

NA.75

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Properties

Product Name

Minimum Essential Medium Eagle, With Earle′s salts, non-essential amino acids, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Quality Segment

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

Endotoxin, tested

components

glucose: 1.0 g/L (Dextro)
L-glutamine: 0.292 g/L
phenol red: 0.011 g/L
Earle’s salts (5% CO₂): yes
NaHCO₃: 2.2 g/L

shipped in

ambient

storage temp.

2-8°C

Description

General description

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Minimum Essential Medium Eagle (MEM) is a synthetic cell culture media, developed by Harry Eagle. It is used for the cultivation of a wide variety of cells grown in monolayers. MEM includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. May be preferred for the growth of adherent cells in the presence of reduced serum or as a base for the development of a serum-free medium.

Application

Minimum Essential Medium Eagle has been used as a culture medium for:

thyroid cancer cell lines
macrophage-like DH82 cells
vero CCL-81 cells

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This Item	M4655	M2414	M2279
Sigma-Aldrich M0325 Minimum Essential Medium Eagle	Sigma-Aldrich M4655 Minimum Essential Medium Eagle	Sigma-Aldrich M2414 Minimum Essential Medium Eagle	Sigma-Aldrich M2279 Minimum Essential Medium Eagle
description With Earle′s salts, non-essential amino acids, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture	description With Earle′s salts, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture	description Modified, with Earle′s salts and reduced NaHCO₃. without L-glutamine, liquid, sterile-filtered, suitable for cell culture	description With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
sterility sterile-filtered	sterility sterile-filtered	sterility sterile-filtered	sterility sterile-filtered
form liquid	form liquid	form liquid	form liquid
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
components glucose: 1.0 g/L (Dextro) L-glutamine: 0.292 g/L phenol red: 0.011 g/L Earle’s salts (5% CO₂): yes NaHCO₃: 2.2 g/L	components sodium pyruvate: no phenol red: yes Earle’s salts (5% CO₂): yes HEPES: no NaHCO₃: yes L-glutamine: yes	components phenol red: 0.011 g/L NaHCO₃: 0.85 g/L glucose: 1.0 g/L L-glutamine: no	components phenol red: yes sodium pyruvate: no L-glutamine: no HEPES: no NaHCO₃: yes Earle’s salts (5% CO₂): yes
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C

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Storage Class

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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