Since certain components in 10X medium concentrates precipitate at pH 7.0, it is necessary to adjust the pH of these solutions to maintain solubility.
Therefore, it may be necessary for the user to adjust the pH of the 1X preparation with sterile 1 N NaOH or 1 N HCl. Procedure For Dilution to 1X (1 Liter):
(NOTE: Dilution of 10X solutions should be performed with sterile containers, components, and equipment.)
• Aseptically measure out approximately 850 ml of tissue culture grade water into an appropriate size container.
• While gently stirring the water, add 100 ml of 10X medium.
• To the solution, add the required amount of sodium bicarbonate ( 0.35 g/L) and L-glutamine (0.1 g/L), at 1X
• While stirring, adjust the solution to desired pH with 1 N NaOH or 1 N HCl.
• Bring medium to final volume with additional tissue culture grade water.
• It is recommended to sterile filter the prepared medium through a 0.22 ?m sterile membrane, or, for example, a Stericup.
• Aseptically dispense into sterile containers. Store refrigerated at 2-8° C
M0275
Minimum Essential Medium Eagle
With Earle′s salts, without ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture, 10x
Synonym(s):
EMEM, MEM
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About This Item
Product Name
Minimum Essential Medium Eagle, 10 ×, With Earle′s salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Quality Segment
sterility
sterile-filtered
form
liquid
concentration
10 ×
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
phenol red: yes
HEPES: no
Earle’s salts (5% CO2): yes
L-glutamine: no
sodium pyruvate: no
NaHCO3: no
shipped in
ambient
storage temp.
2-8°C
Application
Preparation Note
1 of 1
This Item | |||
|---|---|---|---|
| description 10 ×, With Earle′s salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture | description With Earle′s salts, non-essential amino acids and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture | description With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture | description with Earle′s salts and non-essential amino acids, without L-glutamine, phenol red and sodium bicarbonate, Modified, powder, suitable for cell culture |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility - |
| form liquid | form liquid | form liquid | form powder |
| components phenol red: yes | components phenol red: yes | components phenol red: yes | components Earle’s salts (5% CO2): yes |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
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Storage Class
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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If I have 10x Medium, how can I dilute it in order to have 1x and grow cells?
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